Long Non-coding RNA GAPLINC Promotes Angiogenesis by Regulating MiR-211 Under Hypoxia in Human Umbilical Vein Endothelial Cells

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2019 Oct 8

PMID 31589383

Citations 7

Authors

Yangyan He

Ziheng Wu

Chenyang Qiu

Xiaohui Wang

Yilang Xiang

Tian Lu

Yunjun He

Tao Shang

Qianqian Zhu

Xun Wang

Qinglong Zeng

Hongkun Zhang

Donglin Li

Affiliations

Soon will be listed here.

Abstract

In this study, we investigated the role of a long non-coding RNA GAPLINC in angiogenesis using human umbilical vein endothelial cells (HUVEC). We found that hypoxia and hypoxia-inducible factor 1α (HIF-1α) increased the expression of GAPLINC in HUVEC cells. Moreover, GAPLINC overexpression down-regulated miR-211 and up-regulated Bcl2 protein expression. Further rescue experiments confirmed that hypoxia directly increased GAPLINC expression. GAPLINC overexpression also increased cell migration and vessel formation which promoted angiogenesis, and these changes were attributed to the increased expression of vascular endothelial growth factor receptors (VEGFR) and delta-like canonical notch ligand 4 (DLL4) receptors. Finally, we demonstrated that GAPLINC promotes vessel formation and migration by regulating MAPK and NF-kB signalling pathways. Taken together, these findings comprehensively demonstrate that overexpression of GAPLINC increases HUVEC cells angiogenesis under hypoxia condition suggesting that GAPLINC can be a potential target for critical limb ischaemia (CLI) treatment.

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