Bacterial Succession Along a Sediment Porewater Gradient at Lake Neusiedl in Austria

Overview

Journal Sci Data

Specialty Science

Date 2019 Sep 1

PMID 31471542

Citations 7

Authors

Avril Jean Elisabeth von Hoyningen-Huene

Dominik Schneider

Dario Fussmann

Andreas Reimer

Gernot Arp

Rolf Daniel

Affiliations

Soon will be listed here.

Abstract

We provide bacterial 16S rRNA community and hydrochemical data from water and sediments of Lake Neusiedl, Austria. The sediments were retrieved at 5 cm intervals from 30-40 cm push cores. The lake water community was recovered by filtration through a 3.0/0.2 µm filter sandwich. For 16S rRNA gene amplicon-based community profiling, DNA was extracted from the sediment and filters and the bacterial V3-V4 regions were amplified and sequenced using a MiSeq instrument (Illumina). The reads were quality-filtered and processed using open source bioinformatic tools, such as PEAR, cutadapt and VSEARCH. The taxonomy was assigned against the SILVA SSU NR 132 database. The bacterial community structure was visualised in relation to water and porewater chemistry data. The bacterial community in the water column is distinct from the sediment. The most abundant phyla in the sediment shift from Proteobacteria to Chloroflexota (formerly Chloroflexi). Ammonium and total alkalinity increase while sulphate concentrations in the porewater decrease. The provided data are of interest for studies targeting biogeochemical cycling in lake sediments.

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