Chicken CATH-2 Increases Antigen Presentation Markers on Chicken Monocytes and Macrophages

Overview

Journal Protein Pept Lett

Publisher Bentham Science Publishers

Specialty Biochemistry

Date 2019 Aug 1

PMID 31362652

Citations 5

Authors

Marina D Kraaij

Albert van Dijk

Maaike R Scheenstra

Roel M van Harten

Henk P Haagsman

Edwin J A Veldhuizen

Affiliations

Soon will be listed here.

Abstract

Background: Cathelicidins are a family of Host Defense Peptides (HDPs), that play an important role in the innate immune response. They exert both broad-spectrum antimicrobial activity against pathogens, and strong immunomodulatory functions that affect the response of innate and adaptive immune cells.

Objective: The aim of this study was to investigate immunomodulation by the chicken cathelicidin CATH-2 and compare its activities to those of the human cathelicidin LL-37.

Methods: Chicken macrophages and chicken monocytes were incubated with cathelicidins. Activation of immune cells was determined by measuring surface markers Mannose Receptor Ctype 1 (MRC1) and MHC-II. Cytokine production was measured by qPCR and nitric oxide production was determined using the Griess assay. Finally, the effect of cathelicidins on phagocytosis was measured using carboxylate-modified polystyrene latex beads.

Results: CATH-2 and its all-D enantiomer D-CATH-2 increased MRC1 and MHC-II expression, markers for antigen presentation, on primary chicken monocytes, whereas LL-37 did not. D-CATH- 2 also increased the MRC1 and MHC-II expression if a chicken macrophage cell line (HD11 cells) was used. In addition, LPS-induced NO production by HD11 cells was inhibited by CATH-2 and D-CATH-2.

Conclusion: These results are a clear indication that CATH-2 (and D-CATH-2) affect the activation state of monocytes and macrophages, which leads to optimization of the innate immune response and enhancement of the adaptive immune response.

Citing Articles

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Peng L, Tian H, Lu Y, Jia K, Ran J, Tao Q Vet Res. 2022; 53(1):69.

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References

Brown K, Poon G, Birkenhead D, Pena O, Falsafi R, Dahlgren C . Host defense peptide LL-37 selectively reduces proinflammatory macrophage responses. J Immunol. 2011; 186(9):5497-505. DOI: 10.4049/jimmunol.1002508. View

Beaumont P, McHugh B, Gwyer Findlay E, Mackellar A, Mackenzie K, Gallo R . Cathelicidin host defence peptide augments clearance of pulmonary Pseudomonas aeruginosa infection by its influence on neutrophil function in vivo. PLoS One. 2014; 9(6):e99029. PMC: 4041793. DOI: 10.1371/journal.pone.0099029. View

Cuperus T, van Dijk A, Matthijs M, Veldhuizen E, Haagsman H . Protective effect of in ovo treatment with the chicken cathelicidin analog D-CATH-2 against avian pathogenic E. coli. Sci Rep. 2016; 6:26622. PMC: 4882517. DOI: 10.1038/srep26622. View

Berczi I, Bertok L, Bereznai T . Comparative studies on the toxicity of Escherichia coli lipopolysaccharide endotoxin in various animal species. Can J Microbiol. 1966; 12(5):1070-1. DOI: 10.1139/m66-143. View

Bommineni Y, Pham G, Sunkara L, Achanta M, Zhang G . Immune regulatory activities of fowlicidin-1, a cathelicidin host defense peptide. Mol Immunol. 2014; 59(1):55-63. DOI: 10.1016/j.molimm.2014.01.004. View

Cuperus T, Coorens M, van Dijk A, Haagsman H . Avian host defense peptides. Dev Comp Immunol. 2013; 41(3):352-69. DOI: 10.1016/j.dci.2013.04.019. View

Alkie T, Taha-Abdelaziz K, Barjesteh N, Bavananthasivam J, Hodgins D, Sharif S . Characterization of Innate Responses Induced by PLGA Encapsulated- and Soluble TLR Ligands In Vitro and In Vivo in Chickens. PLoS One. 2017; 12(1):e0169154. PMC: 5207720. DOI: 10.1371/journal.pone.0169154. View

Veldhuizen E, Brouwer E, Schneider V, Fluit A . Chicken cathelicidins display antimicrobial activity against multiresistant bacteria without inducing strong resistance. PLoS One. 2013; 8(4):e61964. PMC: 3632573. DOI: 10.1371/journal.pone.0061964. View

van Dijk A, van Eldik M, Veldhuizen E, Tjeerdsma-van Bokhoven H, de Zoete M, Bikker F . Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides. PLoS One. 2016; 11(2):e0147919. PMC: 4743981. DOI: 10.1371/journal.pone.0147919. View

10.

Goitsuka R, Chen C, Benyon L, Asano Y, Kitamura D, Cooper M . Chicken cathelicidin-B1, an antimicrobial guardian at the mucosal M cell gateway. Proc Natl Acad Sci U S A. 2007; 104(38):15063-8. PMC: 1986613. DOI: 10.1073/pnas.0707037104. View

11.

Benincasa M, Scocchi M, Pacor S, Tossi A, Nobili D, Basaglia G . Fungicidal activity of five cathelicidin peptides against clinically isolated yeasts. J Antimicrob Chemother. 2006; 58(5):950-9. DOI: 10.1093/jac/dkl382. View

12.

Bommineni Y, Achanta M, Alexander J, Sunkara L, Ritchey J, Zhang G . A fowlicidin-1 analog protects mice from lethal infections induced by methicillin-resistant Staphylococcus aureus. Peptides. 2010; 31(7):1225-30. DOI: 10.1016/j.peptides.2010.03.037. View

13.

Ciornei C, Sigurdardottir T, Schmidtchen A, Bodelsson M . Antimicrobial and chemoattractant activity, lipopolysaccharide neutralization, cytotoxicity, and inhibition by serum of analogs of human cathelicidin LL-37. Antimicrob Agents Chemother. 2005; 49(7):2845-50. PMC: 1168709. DOI: 10.1128/AAC.49.7.2845-2850.2005. View

14.

Kraaij M, van Dijk A, Haagsman H . CATH-2 and LL-37 increase mannose receptor expression, antigen presentation and the endocytic capacity of chicken mononuclear phagocytes. Mol Immunol. 2017; 90:118-125. DOI: 10.1016/j.molimm.2017.07.005. View

15.

Xiao Y, Cai Y, Bommineni Y, Fernando S, Prakash O, Gilliland S . Identification and functional characterization of three chicken cathelicidins with potent antimicrobial activity. J Biol Chem. 2005; 281(5):2858-67. DOI: 10.1074/jbc.M507180200. View

16.

Coorens M, van Dijk A, Bikker F, Veldhuizen E, Haagsman H . Importance of Endosomal Cathelicidin Degradation To Enhance DNA-Induced Chicken Macrophage Activation. J Immunol. 2015; 195(8):3970-7. DOI: 10.4049/jimmunol.1501242. View

17.

Zanetti M . The role of cathelicidins in the innate host defenses of mammals. Curr Issues Mol Biol. 2005; 7(2):179-96. View

18.

Bhunia A, Mohanram H, Bhattacharjya S . Lipopolysaccharide bound structures of the active fragments of fowlicidin-1, a cathelicidin family of antimicrobial and antiendotoxic peptide from chicken, determined by transferred nuclear Overhauser effect spectroscopy. Biopolymers. 2008; 92(1):9-22. DOI: 10.1002/bip.21104. View

19.

Choi K, Chow L, Mookherjee N . Cationic host defence peptides: multifaceted role in immune modulation and inflammation. J Innate Immun. 2012; 4(4):361-70. PMC: 6741489. DOI: 10.1159/000336630. View

20.

Veldhuizen E, Scheenstra M, Tjeerdsma-van Bokhoven J, Coorens M, Schneider V, Bikker F . Antimicrobial and Immunomodulatory Activity of PMAP-23 Derived Peptides. Protein Pept Lett. 2017; 24(7):609-616. DOI: 10.2174/0929866524666170428150925. View