High-throughput Analysis of the Human Thymic Vδ1 T Cell Receptor Repertoire

Overview

Journal Sci Data

Specialty Science

Date 2019 Jul 6

PMID 31273218

Citations 19

Authors

Biagio Di Lorenzo

Sarina Ravens

Bruno Silva-Santos

Affiliations

Soon will be listed here.

Abstract

γδ T cells are a relatively rare subset of lymphocytes in the human peripheral blood, but they play important roles at the interface between the innate and the adaptive immune systems. The γδ T cell lineage is characterized by a signature γδ T cell receptor (γδTCR) that displays extensive sequence variability originated by DNA rearrangement of the corresponding V(D)J loci. Human γδ T cells comprise Vγ9Vδ2 T cells, the major subset in the peripheral blood; and Vδ1 T cells, the predominant subpopulation in the post-natal thymus and in peripheral tissues. While less studied, Vδ1 T cells recently gathered significant attention due to their anti-cancer and anti-viral activities. In this study we applied next-generation sequencing (NGS) to analyse the γδTCR repertoire of highly (FACS-)purified Vδ1 T cells from human thymic biopsies. Our analysis reveals unsuspected aspects of thymically rearranged and expressed (at the mRNA level) TRG and TRD genes, thus constituting a data resource that qualifies previous conclusions on the TCR repertoire of γδ T cells developing in the human thymus.

Citing Articles

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