Clonal Selection in the Human Vδ1 T Cell Repertoire Indicates γδ TCR-dependent Adaptive Immune Surveillance

Overview

Journal Nat Commun

Specialty Biology

Date 2017 Mar 2

PMID 28248310

Citations 149

Authors

Martin S Davey

Carrie R Willcox

Stephen P Joyce

Kristin Ladell

Sofya A Kasatskaya

James E McLaren

Stuart Hunter

Mahboob Salim

Fiyaz Mohammed

David A Price

Dmitriy M Chudakov

Benjamin E Willcox

Affiliations

Soon will be listed here.

Abstract

γδ T cells are considered to be innate-like lymphocytes that respond rapidly to stress without clonal selection and differentiation. Here we use next-generation sequencing to probe how this paradigm relates to human Vδ2 T cells, implicated in responses to viral infection and cancer. The prevalent Vδ1 T cell receptor (TCR) repertoire is private and initially unfocused in cord blood, typically becoming strongly focused on a few high-frequency clonotypes by adulthood. Clonal expansions have differentiated from a naive to effector phenotype associated with CD27 downregulation, retaining proliferative capacity and TCR sensitivity, displaying increased cytotoxic markers and altered homing capabilities, and remaining relatively stable over time. Contrastingly, Vδ2 T cells express semi-invariant TCRs, which are present at birth and shared between individuals. Human Vδ1 T cells have therefore evolved a distinct biology from the Vδ2 subset, involving a central, personalized role for the γδ TCR in directing a highly adaptive yet unconventional form of immune surveillance.

Citing Articles

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