IMGT/HighV QUEST Paradigm for T Cell Receptor IMGT Clonotype Diversity and Next Generation Repertoire Immunoprofiling

Overview

Journal Nat Commun

Specialty Biology

Date 2013 Sep 3

PMID 23995877

Citations 130

Authors

Shuo Li

Marie-Paule Lefranc

John J Miles

Eltaf Alamyar

Veronique Giudicelli

Patrice Duroux

J Douglas Freeman

Vincent D A Corbin

Jean-Pierre Scheerlinck

Michael A Frohman

Paul U Cameron

Magdalena Plebanski

Bruce Loveland

Scott R Burrows

Anthony T Papenfuss

Eric J Gowans

Affiliations

Soon will be listed here.

Abstract

T cell repertoire diversity and clonotype follow-up in vaccination, cancer, infectious and immune diseases represent a major challenge owing to the enormous complexity of the data generated. Here we describe a next generation methodology, which combines 5'RACE PCR, 454 sequencing and, for analysis, IMGT, the international ImMunoGeneTics information system (IMGT), IMGT/HighV-QUEST web portal and IMGT-ONTOLOGY concepts. The approach is validated in a human case study of T cell receptor beta (TRB) repertoire, by chronologically tracking the effects of influenza vaccination on conventional and regulatory T cell subpopulations. The IMGT/HighV-QUEST paradigm defines standards for genotype/haplotype analysis and characterization of IMGT clonotypes for clonal diversity and expression and achieves a degree of resolution for next generation sequencing verifiable by the user at the sequence level, while providing a normalized reference immunoprofile for human TRB.

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