Analyses of Biomarkers of Exposure to Nephrotoxic Mycotoxins in a Cohort of Patients with Renal Tumours

Overview

Journal Mycotoxin Res

Specialty Microbiology

Date 2019 Jun 30

PMID 31254204

Citations 15

Authors

Frantisek Malir

Miroslav Louda

Vladimir Ostry

Jakub Toman

Nurshad Ali

Yann Grosse

Eva Malirova

Jaroslav Pacovsky

Darina Pickova

Milos Brodak

Annie Pfohl-Leszkowicz

Gisela H Degen

Affiliations

Soon will be listed here.

Abstract

The Czech Republic occupies the first place in the world in the frequency of renal and other urinary tract tumours, but their aetiology is unknown. To explore whether carcinogenic and nephrotoxic mycotoxins may contribute to kidney diseases in the Czech population, biomarkers of ochratoxin A (OTA) and citrinin (CIT) exposure were determined in biological specimens from a cohort of 50 patients with malignant renal tumours. Biomarker analyses in blood and urine samples used validated targeted methods for measuring OTA and CIT plus dihydrocitrinone (DH-CIT) after enrichment of analytes by specific immunoaffinity clean-up. OTA and CIT plus its metabolite DH-CIT were frequently detected in patient urine samples (OTA 62%; CIT 91%; DH-CIT 100%). The concentration ranges in urine were 1-27.8 ng/L for OTA, 2-87 ng/L for CIT and 2-160 ng/L for DH-CIT. The analyses of blood samples revealed also a frequent co-occurrence of OTA and CIT, in the ranges of 40-870 ng/L serum for OTA and 21-182 ng/L plasma for CIT. This first analysis of biomarkers in blood and urine samples of Czech patients revealed no major differences in comparison with published data for the general healthy Czech and European populations. Nonetheless, a frequent co-occurrence of CIT and OTA biomarkers in patient samples may be of interest with regard to potential interactions with other risk factors for renal disease.

Citing Articles

Holocellulose from a Winemaking By-Product to Develop a Biopolymeric System for Bacterial Immobilization: Adsorption of Ochratoxin A in Wine Model Solutions (Box-Behnken Design).

Carrasco-Sanchez V, Laurie V, Munoz-Vera M, Castro R Toxins (Basel). 2025; 17(1.

PMID: 39852979 PMC: 11768956. DOI: 10.3390/toxins17010026.

Effect of Bioactive Ingredients on Urinary Excretion of Aflatoxin B1 and Ochratoxin A in Rats, as Measured by Liquid Chromatography with Fluorescence Detection.

Vila-Donat P, Sanchez D, Cimbalo A, Manes J, Manyes L Toxins (Basel). 2024; 16(8).

PMID: 39195773 PMC: 11359235. DOI: 10.3390/toxins16080363.

High-Coverage UHPLC-MS/MS Analysis of 67 Mycotoxins in Plasma for Male Infertility Exposure Studies.

Ning X, Wang L, Wang J, Ji J, Jin S, Sun J Toxics. 2024; 12(6).

PMID: 38922075 PMC: 11209182. DOI: 10.3390/toxics12060395.

Mycotoxins in Cereal-Based Products and Their Impacts on the Health of Humans, Livestock Animals and Pets.

Yu J, Pedroso I Toxins (Basel). 2023; 15(8).

PMID: 37624237 PMC: 10467131. DOI: 10.3390/toxins15080480.

New perspectives in application of kidney biomarkers in mycotoxin induced nephrotoxicity, with a particular focus on domestic pigs.

Raduly Z, Szabo A, Mezes M, Balatoni I, Price R, Dockrell M Front Microbiol. 2023; 14:1085818.

PMID: 37125184 PMC: 10140568. DOI: 10.3389/fmicb.2023.1085818.

References

Ringot D, Chango A, Schneider Y, Larondelle Y . Toxicokinetics and toxicodynamics of ochratoxin A, an update. Chem Biol Interact. 2005; 159(1):18-46. DOI: 10.1016/j.cbi.2005.10.106. View

Gekle M, Sauvant C, Schwerdt G . Ochratoxin A at nanomolar concentrations: a signal modulator in renal cells. Mol Nutr Food Res. 2005; 49(2):118-30. DOI: 10.1002/mnfr.200400062. View

Miraglia , Brera , Colatosti . Application of Biomarkers to Assessment of Risk to Human Health from Exposure to Mycotoxins. Microchem J. 1996; 54(4):472-7. DOI: 10.1006/mchj.1996.0124. View

Peraica M, Domijan A, Saric M . Mycotoxic and aristolochic acid theories of the development of endemic nephropathy. Arh Hig Rada Toksikol. 2008; 59(1):59-65. DOI: 10.2478/10004-1254-59-2008-1865. View

Fromme H, Gareis M, Volkel W, Gottschalk C . Overall internal exposure to mycotoxins and their occurrence in occupational and residential settings--An overview. Int J Hyg Environ Health. 2016; 219(2):143-65. DOI: 10.1016/j.ijheh.2015.11.004. View

Malir F, Ostry V, Pfohl-Leszkowicz A, Roubal T . Ochratoxin A exposure biomarkers in the Czech Republic and comparison with foreign countries. Biomarkers. 2012; 17(7):577-89. DOI: 10.3109/1354750X.2012.692392. View

Ali N, Hossain K, Degen G . Blood plasma biomarkers of citrinin and ochratoxin A exposure in young adults in Bangladesh. Mycotoxin Res. 2017; 34(1):59-67. DOI: 10.1007/s12550-017-0299-5. View

Duarte S, Pena A, Lino C . Human ochratoxin a biomarkers--from exposure to effect. Crit Rev Toxicol. 2011; 41(3):187-212. DOI: 10.3109/10408444.2010.529103. View

. [Standardization of substance contents in urine--creatinine. Statement of the Commission "Human Biomonitoring" of the Environmental Agency]. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2005; 48(5):616-8. DOI: 10.1007/s00103-005-1029-2. View

10.

Degen G, Ali N, Gundert-Remy U . Preliminary data on citrinin kinetics in humans and their use to estimate citrinin exposure based on biomarkers. Toxicol Lett. 2017; 282:43-48. DOI: 10.1016/j.toxlet.2017.10.006. View

11.

Ali N, Munoz K, Degen G . Ochratoxin A and its metabolites in urines of German adults-An assessment of variables in biomarker analysis. Toxicol Lett. 2017; 275:19-26. DOI: 10.1016/j.toxlet.2017.04.013. View

12.

Gilbert J, Brereton P, MacDonald S . Assessment of dietary exposure to ochratoxin A in the UK using a duplicate diet approach and analysis of urine and plasma samples. Food Addit Contam. 2002; 18(12):1088-93. DOI: 10.1080/02652030110070030. View

13.

Turner P, Flannery B, Isitt C, Ali M, Pestka J . The role of biomarkers in evaluating human health concerns from fungal contaminants in food. Nutr Res Rev. 2012; 25(1):162-79. DOI: 10.1017/S095442241200008X. View

14.

Blaszkewicz M, Munoz K, Degen G . Methods for analysis of citrinin in human blood and urine. Arch Toxicol. 2013; 87(6):1087-94. DOI: 10.1007/s00204-013-1010-z. View

15.

Ali N, Blaszkewicz M, Mohanto N, Rahman M, Alim A, Hossain K . First results on citrinin biomarkers in urines from rural and urban cohorts in Bangladesh. Mycotoxin Res. 2014; 31(1):9-16. DOI: 10.1007/s12550-014-0217-z. View

16.

Munoz K, Blaszkewicz M, Campos V, Vega M, Degen G . Exposure of infants to ochratoxin A with breast milk. Arch Toxicol. 2013; 88(3):837-46. DOI: 10.1007/s00204-013-1168-4. View

17.

Ostry V, Malir F, Dofkova M, Skarkova J, Pfohl-Leszkowicz A, Ruprich J . Ochratoxin A Dietary Exposure of Ten Population Groups in the Czech Republic: Comparison with Data over the World. Toxins (Basel). 2015; 7(9):3608-35. PMC: 4591665. DOI: 10.3390/toxins7093608. View

18.

Ali N, Blaszkewicz M, Degen G . Occurrence of the mycotoxin citrinin and its metabolite dihydrocitrinone in urines of German adults. Arch Toxicol. 2014; 89(4):573-8. DOI: 10.1007/s00204-014-1363-y. View

19.

Zimmerli B, Dick R . Determination of ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high-performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column cleanup: methodology and Swiss data. J Chromatogr B Biomed Appl. 1995; 666(1):85-99. DOI: 10.1016/0378-4347(94)00569-q. View

20.

Ali N, Blaszkewicz M, Alim A, Hossain K, Degen G . Urinary biomarkers of ochratoxin A and citrinin exposure in two Bangladeshi cohorts: follow-up study on regional and seasonal influences. Arch Toxicol. 2015; 90(11):2683-2697. DOI: 10.1007/s00204-015-1654-y. View