Effect of Sulforaphane and 5-Aza-2'-Deoxycytidine on Melanoma Cell Growth

Overview

Journal Medicines (Basel)

Specialty Pharmacology

Date 2019 Jun 30

PMID 31252639

Citations 6

Authors

Tung-Chin Chiang

Brian Koss

L Joseph Su

Charity L Washam

Stephanie D Byrum

Aaron Storey

Alan J Tackett

Affiliations

Soon will be listed here.

Abstract

UV exposure-induced oxidative stress is implicated as a driving mechanism for melanoma. Increased oxidative stress results in DNA damage and epigenetic dysregulation. Accordingly, we explored whether a low dose of the antioxidant sulforaphane (SFN) in combination with the epigenetic drug 5-aza-2'-deoxycytidine (DAC) could slow melanoma cell growth. SFN is a natural bioactivated product of the cruciferous family, while DAC is a DNA methyltransferase inhibitor. Melanoma cell growth characteristics, gene transcription profiles, and histone epigenetic modifications were measured after single and combination treatments with SFN and DAC. We detected melanoma cell growth inhibition and specific changes in gene expression profiles upon combinational treatments with SFN and DAC, while no significant alterations in histone epigenetic modifications were observed. Dysregulated gene transcription of a key immunoregulator cytokine-C-C motif ligand 5 (CCL-5)-was validated. These results indicate a potential combinatorial effect of a dietary antioxidant and an FDA-approved epigenetic drug in controlling melanoma cell growth.

Citing Articles

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