» Articles » PMID: 31222043

Reducing Campylobacter Jejuni Colonization in Broiler Chickens by In-feed Supplementation with Hyperimmune Egg Yolk Antibodies

Overview
Journal Sci Rep
Specialty Science
Date 2019 Jun 22
PMID 31222043
Citations 19
Authors
Affiliations
Soon will be listed here.
Abstract

Campylobacter infections sourced mainly to poultry products, are the most important bacterial foodborne zoonoses worldwide. No effective measures to control these infections in broiler production exist to date. Here, we used passive immunization with hyperimmune egg yolks to confer broad protection of broilers against Campylobacter infection. Two novel vaccines, a bacterin of thirteen Campylobacter jejuni (C. jejuni) and C. coli strains and a subunit vaccine of six immunodominant Campylobacter antigens, were used for the immunization of layers, resulting in high and prolonged levels of specific immunoglobulin Y (IgY) in the hens' yolks. In the first in vivo trial, yolks (sham, bacterin or subunit vaccine derived) were administered prophylactically in the broiler feed. Both the bacterin- and subunit vaccine-induced IgY significantly reduced the number of Campylobacter-colonized broilers. In the second in vivo trial, the yolks were administered therapeutically during three days before euthanasia. The bacterin IgY resulted in a significant decrease in C. jejuni counts per infected bird. The hyperimmune yolks showed strong reactivity to a broad representation of C. jejuni and C. coli clonal complexes. These results indicate that passive immunization with hyperimmune yolks, especially bacterin derived, offers possibilities to control Campylobacter colonization in poultry.

Citing Articles

A multi-antigen Campylobacter vaccine enhances antibody responses in layer breeders and sustains elevated maternal antibody levels in their offspring.

Naguib M, Sharma S, Schneider A, Bragg A, Abdelaziz K Poult Sci. 2025; 104(4):104898.

PMID: 39985894 PMC: 11904490. DOI: 10.1016/j.psj.2025.104898.


Efficacy of hyperimmunized egg yolk antibodies (IgY) against Campylobacter jejuni: In Vitro and In Vivo evaluations.

Soltani N, Rahimi S, Khaki P, Karimi Torshizi M, Eskandari B, Grimes J Poult Sci. 2025; 104(2):104718.

PMID: 39787828 PMC: 11761915. DOI: 10.1016/j.psj.2024.104718.


Application of a liposomal subunit vaccine in chickens for reduction of gut colonisation.

Lasica A, Godlewska R, Gubernator J, Jakubiak-Augustyn A, Majewski P, Wyszynska A J Vet Res. 2025; 68(4):487-496.

PMID: 39776683 PMC: 11702242. DOI: 10.2478/jvetres-2024-0062.


Effect of dietary medicinal plants on physiological responses of broiler chickens challenged with Campylobacter jejuni.

Nazari Z, Shirzadi H, Taherpour K, Rahmatnejad E, Khatibjoo A Vet Med Sci. 2024; 10(5):e70028.

PMID: 39258513 PMC: 11388060. DOI: 10.1002/vms3.70028.


Long-Term Oral Administration of Hyperimmune Egg-Based IgY-Rich Formulations Induces Mucosal Immune Response and Systemic Increases of Cytokines Involved in Th2- and Th17-Type Immune Responses in C57BL/6 Mice.

Nastasa V, Minea B, Pasca A, Bostanaru-Iliescu A, Stefan A, Gologan D Int J Mol Sci. 2024; 25(16).

PMID: 39201385 PMC: 11354499. DOI: 10.3390/ijms25168701.


References
1.
Lee S, Lillehoj H, Park D, Jang S, Morales A, Garcia D . Induction of passive immunity in broiler chickens against Eimeria acervulina by hyperimmune egg yolk immunoglobulin Y. Poult Sci. 2009; 88(3):562-6. DOI: 10.3382/ps.2008-00340. View

2.
Nachamkin I . Chronic effects of Campylobacter infection. Microbes Infect. 2002; 4(4):399-403. DOI: 10.1016/s1286-4579(02)01553-8. View

3.
Tsubokura K, Berndtson E, Bogstedt A, Kaijser B, Kim M, Ozeki M . Oral administration of antibodies as prophylaxis and therapy in Campylobacter jejuni-infected chickens. Clin Exp Immunol. 1997; 108(3):451-5. PMC: 1904686. DOI: 10.1046/j.1365-2249.1997.3901288.x. View

4.
Hermans D, Van Steendam K, Verbrugghe E, Verlinden M, Martel A, Seliwiorstow T . Passive immunization to reduce Campylobacter jejuni colonization and transmission in broiler chickens. Vet Res. 2014; 45:27. PMC: 3996517. DOI: 10.1186/1297-9716-45-27. View

5.
Suzuki H, Yamamoto S . Campylobacter contamination in retail poultry meats and by-products in the world: a literature survey. J Vet Med Sci. 2009; 71(3):255-61. DOI: 10.1292/jvms.71.255. View