Identification and Purification of a Polypeptide That Binds to the C-fos Serum Response Element

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1987 Sep 1

PMID 3119326

Citations 157

Authors

R Treisman

Affiliations

Soon will be listed here.

Abstract

A short DNA sequence element, the serum response element (SRE), which binds a nuclear protein, serum response factor (SRF), mediates transient transcriptional activation of c-fos and cytoskeletal actin genes in response to serum factors. Variant SRE sequences with different affinities for HeLa cell SRF were synthesised. Binding of SRF to these sites in vitro correlates with the transcriptional properties of these elements in vivo, suggesting that SRF is a positively acting transcription factor. A 67-kd polypeptide was identified as the DNA-binding component of SRF by photoactivated DNA-protein cross-linking in vitro. The high affinity SRF-binding site was used to purify this polypeptide to virtual homogeneity in a single DNA affinity chromatography step.

Citing Articles

Actin-microtubule cytoskeletal interplay mediated by MRTF-A/SRF signaling promotes dilated cardiomyopathy caused by LMNA mutations.

Le Dour C, Chatzifrangkeskou M, Macquart C, Magiera M, Peccate C, Jouve C Nat Commun. 2022; 13(1):7886.

PMID: 36550158 PMC: 9780334. DOI: 10.1038/s41467-022-35639-x.

Old blood from heterochronic parabionts accelerates vascular aging in young mice: transcriptomic signature of pathologic smooth muscle remodeling.

Kiss T, Nyul-Toth A, Gulej R, Tarantini S, Csipo T, Mukli P Geroscience. 2022; 44(2):953-981.

PMID: 35124764 PMC: 9135944. DOI: 10.1007/s11357-022-00519-1.

Landscape of DNA binding signatures of myocyte enhancer factor-2B reveals a unique interplay of base and shape readout.

Dantas Machado A, Cooper B, Lei X, Di Felice R, Chen L, Rohs R Nucleic Acids Res. 2020; 48(15):8529-8544.

PMID: 32738045 PMC: 7470950. DOI: 10.1093/nar/gkaa642.

Reduced NGF in Gastric Endothelial Cells Is One of the Main Causes of Impaired Angiogenesis in Aging Gastric Mucosa.

Ahluwalia A, Jones M, Hoa N, Zhu E, Brzozowski T, Tarnawski A Cell Mol Gastroenterol Hepatol. 2018; 6(2):199-213.

PMID: 29992182 PMC: 6037903. DOI: 10.1016/j.jcmgh.2018.05.003.

Coordination of microtubule acetylation and the actin cytoskeleton by formins.

Fernandez-Barrera J, Alonso M Cell Mol Life Sci. 2018; 75(17):3181-3191.

PMID: 29947928 PMC: 11105221. DOI: 10.1007/s00018-018-2855-3.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Mohun T, Garrett N, Treisman R . Xenopus cytoskeletal actin and human c-fos gene promoters share a conserved protein-binding site. EMBO J. 1987; 6(3):667-73. PMC: 553449. DOI: 10.1002/j.1460-2075.1987.tb04806.x. View

Ogata R, Gilbert W . Contacts between the lac repressor and the thymines in the lac operator. Proc Natl Acad Sci U S A. 1977; 74(11):4973-6. PMC: 432080. DOI: 10.1073/pnas.74.11.4973. View

Oren M, Winocour E, Prives C . Differential affinities of simian virus 40 large tumor antigen for DNA. Proc Natl Acad Sci U S A. 1980; 77(1):220-4. PMC: 348240. DOI: 10.1073/pnas.77.1.220. View

Herrick G . Site-specific DNA-affinity chromatography of the lac repressor. Nucleic Acids Res. 1980; 8(16):3721-8. PMC: 324186. DOI: 10.1093/nar/8.16.3721. View

Garner M, Revzin A . A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res. 1981; 9(13):3047-60. PMC: 327330. DOI: 10.1093/nar/9.13.3047. View

Fried M, Crothers D . Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res. 1981; 9(23):6505-25. PMC: 327619. DOI: 10.1093/nar/9.23.6505. View

Dignam J, Lebovitz R, Roeder R . Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983; 11(5):1475-89. PMC: 325809. DOI: 10.1093/nar/11.5.1475. View

Cochran B, Reffel A, Stiles C . Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell. 1983; 33(3):939-47. DOI: 10.1016/0092-8674(83)90037-5. View

10.

Kelly K, Cochran B, Stiles C, Leder P . Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983; 35(3 Pt 2):603-10. DOI: 10.1016/0092-8674(83)90092-2. View

11.

Strauss F, Varshavsky A . A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome. Cell. 1984; 37(3):889-901. DOI: 10.1016/0092-8674(84)90424-0. View

12.

Pabo C, Sauer R . Protein-DNA recognition. Annu Rev Biochem. 1984; 53:293-321. DOI: 10.1146/annurev.bi.53.070184.001453. View

13.

Greenberg M, Ziff E . Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature. 1984; 311(5985):433-8. DOI: 10.1038/311433a0. View

14.

Cochran B, Zullo J, Verma I, Stiles C . Expression of the c-fos gene and of an fos-related gene is stimulated by platelet-derived growth factor. Science. 1984; 226(4678):1080-2. DOI: 10.1126/science.6093261. View

15.

Elder P, Schmidt L, Ono T, Getz M . Specific stimulation of actin gene transcription by epidermal growth factor and cycloheximide. Proc Natl Acad Sci U S A. 1984; 81(23):7476-80. PMC: 392169. DOI: 10.1073/pnas.81.23.7476. View

16.

Kruijer W, Cooper J, Hunter T, Verma I . Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein. Nature. 1984; 312(5996):711-6. DOI: 10.1038/312711a0. View

17.

Muller R, Bravo R, Burckhardt J, Curran T . Induction of c-fos gene and protein by growth factors precedes activation of c-myc. Nature. 1984; 312(5996):716-20. DOI: 10.1038/312716a0. View

18.

Jones K, Yamamoto K, Tjian R . Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro. Cell. 1985; 42(2):559-72. DOI: 10.1016/0092-8674(85)90113-8. View

19.

Treisman R . Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences. Cell. 1985; 42(3):889-902. DOI: 10.1016/0092-8674(85)90285-5. View

20.

Carthew R, Chodosh L, Sharp P . An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell. 1985; 43(2 Pt 1):439-48. DOI: 10.1016/0092-8674(85)90174-6. View