[Effect of Sclerostin on the Functions and Related Mechanisms of Cementoblasts Under Mechanical Stress]

Overview

Journal Hua Xi Kou Qiang Yi Xue Za Zhi

Specialty Dentistry

Date 2019 Jun 7

PMID 31168982

Citations 4

Authors

Si-Yu Bai

Yue Chen

Hong-Wei Dai

Lan Huang

Affiliations

Soon will be listed here.

Abstract

Objective: The purpose of this study is to investigate the potential effects of sclerostin (SOST) on the biological funtions and related mechanisms of cementoblasts under mechanical stress.

Methods: OCCM-30 cells were treated with varying doses of SOST (0, 25, 50, and 100 ng·mL⁻¹) and were loaded with uniaxial compressive stress (2 000 μ strain with a frequency of 0.5 Hz) for six hours. Western blot was utilized to detect the expressions of β-catenin, p-smad1/5/8, and smad1/5/8 proteins. Alkaline phosphatase (ALP) activity was determined, and reverse transcription polymerase chain reaction was used to measure the expressions of runt-related transcription factor 2 (Runx-2), osteocalcin (OCN), bone sialoproteins (BSP), receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) mRNA.

Results: The expression of p-smad 1/5/8 was significantly downregulated with increasing SOST. β-catenin and smad1/5/8 exhibited no difference. ALP activity decreased under mechanical compressive stress with increasing SOST concentrations. Runx-2 expression was reduced with increasing SOST concentrations, and a similar trend was observed for the BSP and OCN expressions. When the SOST concentration was enhanced, RANKL expression gradually increased, whereas the expression of OPG decreased.

Conclusions: Under mechanical comprehensive stress, SOST can adjust the bone morphogenetic protein (BMP) /smad signal pathway. Osteosclerosis inhibits the mineralization of cementoblasts under mechanical compressive stress, which may be achieved by inhibiting the expressions of osteogenesis factors (Runx2, OCN, BSP, and others) and by promoting the ratio of cementoclast-related factors (RANKL/OPG) through BMP signal pathways.

Citing Articles

Effects of low-level laser on the expression of interleukin-6, tumor necrosis factor‑α, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in human periodontal ligament cells.

Tang M, Cui Z, Wang Y, Chen Z, Li W, Zhang C Hua Xi Kou Qiang Yi Xue Za Zhi. 2023; 41(5):521-532.

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Aging effect of osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in human periodontal ligament cells under continuous static pressure.

Wu J, Cui Z, Han Y, Li W Hua Xi Kou Qiang Yi Xue Za Zhi. 2022; 40(6):654-661.

PMID: 36416317 PMC: 9763954. DOI: 10.7518/hxkq.2022.06.005.

Sclerostin is a promising therapeutic target for oral inflammation and regenerative dentistry.

Liao C, Liang S, Wang Y, Zhong T, Liu X J Transl Med. 2022; 20(1):221.

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Fouad-Elhady E, Aglan H, Hassan R, Ahmed H, Sabry G Heliyon. 2020; 6(2):e03341.

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