Aging Effect of Osteoprotegerin and Receptor Activator of Nuclear Factor-κB Ligand Expression in Human Periodontal Ligament Cells Under Continuous Static Pressure

Overview

Journal Hua Xi Kou Qiang Yi Xue Za Zhi

Specialty Dentistry

Date 2022 Nov 23

PMID 36416317

Authors

Jie Wu

Zhanqin Cui

Yu Han

Wenjing Li

Affiliations

Soon will be listed here.

Abstract

Objectives: The expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in human periodontal ligament cells (HPDLCs) was investigated by cell culture under continuous static pressure.

Methods: HPDLCs were primarily cultured by tissue explant method and divided into three groups: group A (13-18 years old), group B (19-29 years old), and group C (30-44 years old). CCK-8 was used to detect the proliferation of HPDLCs. The senescence of HPDLCs was detected by senescence-associated β-galactosidase staining. Cells in the three groups were respectively given 0, 1.5, 3, 6, 12, 24, 48, and 72 h of continuous static pressure . The expression of OPG and RANKL in the supernatant was detected by enzyme-linked immunosorbent assay.

Results: After continuous static pressure for stimulation, the expression of OPG and RANKL changed. The expression of OPG increased with time and age (<0.01). The expression of RANKL increased with time and decreased with age (<0.01). The ratio of OPG/RANKL initially decreased, increased with time, and then continued to rise with age (<0.01).

Conclusions: Aging could increase the expression of OPG and the ratio of OPG/RANKL and decrease the expression of RANKL in HPDLCs under continuous static pressure .

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