Atomic Resolution Cryo-EM Structure of a Native-like CENP-A Nucleosome Aided by an Antibody Fragment

Overview

Journal Nat Commun

Specialty Biology

Date 2019 May 26

PMID 31127102

Citations 44

Authors

Bing-Rui Zhou

K N Sathish Yadav

Mario Borgnia

Jingjun Hong

Baohua Cao

Ada L Olins

Donald E Olins

Yawen Bai

Ping Zhang

Affiliations

Soon will be listed here.

Abstract

Genomic DNA in eukaryotes is organized into chromatin through association with core histones to form nucleosomes, each distinguished by their DNA sequences and histone variants. Here, we used a single-chain antibody fragment (scFv) derived from the anti-nucleosome antibody mAb PL2-6 to stabilize human CENP-A nucleosome containing a native α-satellite DNA and solved its structure by the cryo-electron microscopy (cryo-EM) to 2.6 Å resolution. In comparison, the corresponding cryo-EM structure of the free CENP-A nucleosome could only reach 3.4 Å resolution. We find that scFv binds to a conserved acidic patch on the histone H2A-H2B dimer without perturbing the nucleosome structure. Our results provide an atomic resolution cryo-EM structure of a nucleosome and insight into the structure and function of the CENP-A nucleosome. The scFv approach is applicable to the structural determination of other native-like nucleosomes with distinct DNA sequences.

Citing Articles

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