Quantifying the Local Resolution of Cryo-EM Density Maps

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2013 Nov 12

PMID 24213166

Citations 1146

Authors

Alp Kucukelbir

Fred J Sigworth

Hemant D Tagare

Affiliations

Soon will be listed here.

Abstract

We propose a definition of local resolution for three-dimensional electron cryo-microscopy (cryo-EM) density maps that uses local sinusoidal features. Our algorithm has no free parameters and is applicable to other imaging modalities, including tomography. By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, we report variable resolution across a 4- to 40-Å range.

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