Differential Macrophage Subsets in Muscle Damage Induced by a K49-PLA from Bothrops Jararacussu Venom Modulate the Time Course of the Regeneration Process

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 2019 May 25

PMID 31123944

Citations 5

Authors

Priscila Andrade Raneia E Silva

Adriana da Costa Neves

Cristiani Baldo da Rocha

Ana Maria Moura-da-Silva

Eliana L Faquim-Mauro

Affiliations

Soon will be listed here.

Abstract

Bothrops snakes cause around 80% of snakebites in Brazil, with muscle tissue damage as an important consequence, which may cause dysfunction on the affected limb. Bothropstoxin-I (BthTX-I) from Bothrops jararacussu is a K49-phospholipase A, involved in the injury and envenomation's inflammatory response. Immune system components act in the resolution of tissue damage and regeneration. Thus, macrophages exert a crucial role in the elimination of dead tissue and muscle repair. Here, we studied the cellular influx and presence of classical and alternative macrophages (M1 and M2) during muscle injury induced by BthTX-I and the regeneration process. BthTX-I elicited intense inflammatory response characterized by neutrophil migration, then increased influx of M1 macrophages followed by M2 population that declined, resulting in tissue regeneration. The high expressions of TNF-α and IL6 were changed by increased TGF-β expression after BthTX-I injection, coinciding with the iNOs and arginase expression and the peaks of M1 and M2 macrophages in muscle tissue. A coordinated sequence of PAX7, MyoD, and myogenin expression involved in muscle regenerative process appeared after BthTX-I injection. Together, these results demonstrate a direct correlation between the macrophage subsets, cytokine microenvironment, and the myogenesis process. This information may be useful for new envenomation and muscular dysfunction therapies.

Citing Articles

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production.

Silva L, Gouveia V, Campos G, Dale C, da Palma R, Ligeiro de Oliveira A PLoS Negl Trop Dis. 2024; 18(5):e0012227.

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Intramuscular Bleeding and Formation of Microthrombi during Skeletal Muscle Damage Caused by a Snake Venom Metalloprotease and a Cardiotoxin.

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Mapping the Immune Cell Microenvironment with Spatial Profiling in Muscle Tissue Injected with the Venom of .

de Oliveira A, Pramoonjago P, Rucavado A, Moskaluk C, Silva D, Escalante T Toxins (Basel). 2023; 15(3).

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Regulatory T cells-centered regulatory networks of skeletal muscle inflammation and regeneration.

Chen Z, Lan H, Liao Z, Huang J, Jian X, Hu J Cell Biosci. 2022; 12(1):112.

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Effects of venoms on neutrophil respiratory burst: a major inflammatory function.

El-Benna J, Hurtado-Nedelec M, Gougerot-Pocidalo M, Dang P J Venom Anim Toxins Incl Trop Dis. 2021; 27:e20200179.

PMID: 34249119 PMC: 8237995. DOI: 10.1590/1678-9199-JVATITD-2020-0179.

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