Moving Forward One Step Back at a Time: Reversibility During Homologous Recombination

Overview

Journal Curr Genet

Specialty Genetics

Date 2019 May 25

PMID 31123771

Citations 25

Authors

Aurele Piazza

Wolf-Dietrich Heyer

Affiliations

Soon will be listed here.

Abstract

DNA double-strand breaks are genotoxic lesions whose repair can be templated off an intact DNA duplex through the conserved homologous recombination (HR) pathway. Because it mainly consists of a succession of non-covalent associations of molecules, HR is intrinsically reversible. Reversibility serves as an integral property of HR, exploited and tuned at various stages throughout the pathway with anti- and pro-recombinogenic consequences. Here, we focus on the reversibility of displacement loops (D-loops), a central DNA joint molecule intermediate whose dynamics and regulation have recently been physically probed in somatic S. cerevisiae cells. From homology search to repair completion, we discuss putative roles of D-loop reversibility in repair fidelity and outcome.

Citing Articles

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