The FANCM Ortholog Fml1 Promotes Recombination at Stalled Replication Forks and Limits Crossing over During DNA Double-strand Break Repair

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2008 Oct 15

PMID 18851838

Citations 106

Authors

Weili Sun

Saikat Nandi

Fekret Osman

Jong Sook Ahn

Jovana Jakovleska

Alexander Lorenz

Matthew C Whitby

Affiliations

Soon will be listed here.

Abstract

The Fanconi anemia (FA) core complex promotes the tolerance/repair of DNA damage at stalled replication forks by catalyzing the monoubiquitination of FANCD2 and FANCI. Intriguingly, the core complex component FANCM also catalyzes branch migration of model Holliday junctions and replication forks in vitro. Here we have characterized the ortholog of FANCM in fission yeast Fml1 in order to understand the physiological significance of this activity. We show that Fml1 has at least two roles in homologous recombination-it promotes Rad51-dependent gene conversion at stalled/blocked replication forks and limits crossing over during mitotic double-strand break repair. In vitro Fml1 catalyzes both replication fork reversal and D loop disruption, indicating possible mechanisms by which it can fulfill its pro- and antirecombinogenic roles.

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