The Long Noncoding RNA Pnky Is a Trans-acting Regulator of Cortical Development In Vivo

Overview

Journal Dev Cell

Publisher Cell Press

Specialties Cell Biology
Reproductive Medicine

Date 2019 May 22

PMID 31112699

Citations 35

Authors

Rebecca E Andersen

Sung Jun Hong

Justin J Lim

Miao Cui

Brock A Harpur

Elizabeth Hwang

Ryan N Delgado

Alexander D Ramos

Siyuan John Liu

Benjamin J Blencowe

Daniel A Lim

Affiliations

Soon will be listed here.

Abstract

While it is now appreciated that certain long noncoding RNAs (lncRNAs) have important functions in cell biology, relatively few have been shown to regulate development in vivo, particularly with genetic strategies that establish cis versus trans mechanisms. Pnky is a nuclear-enriched lncRNA that is transcribed divergently from the neighboring proneural transcription factor Pou3f2. Here, we show that conditional deletion of Pnky from the developing cortex regulates the production of projection neurons from neural stem cells (NSCs) in a cell-autonomous manner, altering postnatal cortical lamination. Surprisingly, Pou3f2 expression is not disrupted by deletion of the entire Pnky gene. Moreover, expression of Pnky from a BAC transgene rescues the differential gene expression and increased neurogenesis of Pnky-knockout NSCs, as well as the developmental phenotypes of Pnky-deletion in vivo. Thus, despite being transcribed divergently from a key developmental transcription factor, the lncRNA Pnky regulates development in trans.

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