Maternal Serum Levels of Angiogenic Markers and Markers of Placentation in Pregnancies Conceived with Fresh and Vitrified-warmed Blastocyst Transfer

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2019 May 20

PMID 31104293

Citations 3

Authors

Milan Reljic

Admir Porovic

Affiliations

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Abstract

Purpose: The aim of the study was to compare the levels of angiogenic markers and markers of placentation between pregnancies conceived with fresh (ET) and vitrified-warmed blastocyst transfer (FET).

Methods: Women with singleton pregnancies resulting from fresh ET or FET during the period between 2013 and 2017 were included in this prospective observational study. Fresh ET was performed in a stimulated and FET in natural cycle. At 6-7 weeks of gestation, after ultrasound confirmation of a single gestational sac with a viable embryo, serum levels of free β-hCG, pregnancy-associated plasma protein A (PAPP-A), placental growth factor (PIGF) and fms-like tyrosine kinase (sFlt-1) were measured. Data on the patients' characteristics, pregnancy complications and outcomes were collected from a questionnaire and National Perinatal Information System of Slovenia.

Results: Among 211 pregnancies, 126 were achieved with fresh ET and 85 with FET. There were no significant differences in perinatal outcome, pregnancy complication and PIGF level between the fresh ET and FET group. Women achieving pregnancy with FET had significant higher levels of free β-hCG (40.20 ± 30.62 IU/L vs. 28.74 ± 23.52, p = 0.002), PAPP-A (0.09 ± 0.06 vs. 0.06 ± 0.05 IU/L, p = 0.004) and sFlt-1 (596.19 ± 283.06 vs. 436.53 ± 248.23 pg/L, p < 0.0001) compared to women having conceived with fresh ET. There were no significant differences in the levels of evaluated biomarkers between patients with different pregnancy outcomes and complications.

Conclusion: Levels of angiogenic markers and markers of placentation differ between pregnancies achieved with fresh ET and FET which may reflect altered implantation and early placentation with some forms of assisted reproductive technologies.

Citing Articles

The impact of fresh versus frozen-thawed embryos on maternal serum analyte levels in IVF singleton and twin pregnancies.

Peyser A, Gulersen M, Krantz D, Li X, Bornstein E, Rochelson B J Assist Reprod Genet. 2023; 41(2):473-481.

PMID: 38133878 PMC: 10894779. DOI: 10.1007/s10815-023-03007-3.

Frozen blastocysts: Assessing the importance of day 5/day 6 blastocysts or blastocyst quality.

Jiang Y, Song G, Zhang X, Miao S, Wu X Exp Ther Med. 2022; 23(5):333.

PMID: 35401807 PMC: 8987946. DOI: 10.3892/etm.2022.11262.

The expression of pregnancy-associated plasma protein-A (PAPP-A) in human blastocoel fluid-conditioned media: a proof of concept study.

Kavoussi S, Chen S, Wininger J, Lal A, Roudebush W, Lanford H J Assist Reprod Genet. 2022; 39(2):389-394.

PMID: 35013837 PMC: 8956765. DOI: 10.1007/s10815-022-02393-4.

References

Vlaisavljevic V, Kovacic B, Gavric-Lovrec V, Reljic M . Simplification of the clinical phase of IVF and ICSI treatment in programmed cycles. Int J Gynaecol Obstet. 2000; 69(2):135-42. DOI: 10.1016/s0020-7292(00)00177-6. View

Reis F, DAntona D, Petraglia F . Predictive value of hormone measurements in maternal and fetal complications of pregnancy. Endocr Rev. 2002; 23(2):230-57. DOI: 10.1210/edrv.23.2.0459. View

Hui P, Lam Y, Tang M, Ng E, Yeung W, Ho P . Maternal serum pregnancy-associated plasma protein-A and free beta-human chorionic gonadotrophin in pregnancies conceived with fresh and frozen-thawed embryos from in vitro fertilization and intracytoplasmic sperm injection. Prenat Diagn. 2005; 25(5):390-3. DOI: 10.1002/pd.1169. View

Tul N, Novak-Antolic Z . Serum PAPP-A levels at 10-14 weeks of gestation are altered in women after assisted conception. Prenat Diagn. 2006; 26(13):1206-11. DOI: 10.1002/pd.1589. View

Evans M, Krantz D, Hallahan T, Galen R . Meta-analysis of first trimester Down syndrome screening studies: free beta-human chorionic gonadotropin significantly outperforms intact human chorionic gonadotropin in a multimarker protocol. Am J Obstet Gynecol. 2007; 196(3):198-205. DOI: 10.1016/j.ajog.2006.08.001. View

Morozov V, Ruman J, Kenigsberg D, Moodie G, Brenner S . Natural cycle cryo-thaw transfer may improve pregnancy outcome. J Assist Reprod Genet. 2007; 24(4):119-23. PMC: 3455063. DOI: 10.1007/s10815-006-9100-y. View

Gjerris A, Loft A, Pinborg A, Christiansen M, Tabor A . First-trimester screening markers are altered in pregnancies conceived after IVF/ICSI. Ultrasound Obstet Gynecol. 2008; 33(1):8-17. DOI: 10.1002/uog.6254. View

Amor D, Xu J, Halliday J, Francis I, Healy D, Breheny S . Pregnancies conceived using assisted reproductive technologies (ART) have low levels of pregnancy-associated plasma protein-A (PAPP-A) leading to a high rate of false-positive results in first trimester screening for Down syndrome. Hum Reprod. 2009; 24(6):1330-8. DOI: 10.1093/humrep/dep046. View

Kolibianakis E, Venetis C, Tarlatzis B . Cryopreservation of human embryos by vitrification or slow freezing: which one is better?. Curr Opin Obstet Gynecol. 2009; 21(3):270-4. DOI: 10.1097/GCO.0b013e3283297dd6. View

10.

Shapiro B, Daneshmand S, Garner F, Aguirre M, Hudson C, Thomas S . Similar ongoing pregnancy rates after blastocyst transfer in fresh donor cycles and autologous cycles using cryopreserved bipronuclear oocytes suggest similar viability of transferred blastocysts. Fertil Steril. 2009; 93(1):319-21. DOI: 10.1016/j.fertnstert.2009.07.966. View

11.

Dumoulin J, Land J, Van Montfoort A, Nelissen E, Coonen E, Derhaag J . Effect of in vitro culture of human embryos on birthweight of newborns. Hum Reprod. 2010; 25(3):605-12. DOI: 10.1093/humrep/dep456. View

12.

Engels M, Kooij M, Schats R, Twisk J, Blankenstein M, van Vugt J . First-trimester serum marker distribution in singleton pregnancies conceived with assisted reproduction. Prenat Diagn. 2010; 30(4):372-7. DOI: 10.1002/pd.2495. View

13.

Kalra S, Ratcliffe S, Milman L, Gracia C, Coutifaris C, Barnhart K . Perinatal morbidity after in vitro fertilization is lower with frozen embryo transfer. Fertil Steril. 2010; 95(2):548-53. PMC: 5830094. DOI: 10.1016/j.fertnstert.2010.05.049. View

14.

Herrero L, Martinez M, Garcia-Velasco J . Current status of human oocyte and embryo cryopreservation. Curr Opin Obstet Gynecol. 2011; 23(4):245-50. DOI: 10.1097/GCO.0b013e32834874e2. View

15.

Shapiro B, Daneshmand S, Garner F, Aguirre M, Hudson C, Thomas S . Evidence of impaired endometrial receptivity after ovarian stimulation for in vitro fertilization: a prospective randomized trial comparing fresh and frozen-thawed embryo transfer in normal responders. Fertil Steril. 2011; 96(2):344-8. DOI: 10.1016/j.fertnstert.2011.05.050. View

16.

Kathiresan A, Cruz-Almeida Y, Barrionuevo M, Maxson W, Hoffman D, Weitzman V . Prognostic value of beta-human chorionic gonadotropin is dependent on day of embryo transfer during in vitro fertilization. Fertil Steril. 2011; 96(6):1362-6. DOI: 10.1016/j.fertnstert.2011.09.042. View

17.

Imudia A, Awonuga A, Doyle J, Kaimal A, Wright D, Toth T . Peak serum estradiol level during controlled ovarian hyperstimulation is associated with increased risk of small for gestational age and preeclampsia in singleton pregnancies after in vitro fertilization. Fertil Steril. 2012; 97(6):1374-9. DOI: 10.1016/j.fertnstert.2012.03.028. View

18.

Maheshwari A, Pandey S, Shetty A, Hamilton M, Bhattacharya S . Obstetric and perinatal outcomes in singleton pregnancies resulting from the transfer of frozen thawed versus fresh embryos generated through in vitro fertilization treatment: a systematic review and meta-analysis. Fertil Steril. 2012; 98(2):368-77.e1-9. DOI: 10.1016/j.fertnstert.2012.05.019. View

19.

Tomas C, Alsbjerg B, Martikainen H, Humaidan P . Pregnancy loss after frozen-embryo transfer--a comparison of three protocols. Fertil Steril. 2012; 98(5):1165-9. DOI: 10.1016/j.fertnstert.2012.07.1058. View

20.

Roque M, Lattes K, Serra S, Sola I, Geber S, Carreras R . Fresh embryo transfer versus frozen embryo transfer in in vitro fertilization cycles: a systematic review and meta-analysis. Fertil Steril. 2012; 99(1):156-162. DOI: 10.1016/j.fertnstert.2012.09.003. View