Celiac Disease-associated Neisseria Flavescens Decreases Mitochondrial Respiration in CaCo-2 Epithelial Cells: Impact of Lactobacillus Paracasei CBA L74 on Bacterial-induced Cellular Imbalance

Overview

Journal Cell Microbiol

Publisher Wiley

Specialties Cell Biology
Microbiology

Date 2019 May 2

PMID 31042331

Citations 15

Authors

Giuseppe Labruna

Merlin Nanayakkara

Chiara Pagliuca

Marcella Nunziato

Laura Iaffaldano

Valeria DArgenio

Roberta Colicchio

Andrea L Budelli

Roberto Nigro

Paola Salvatore

Maria Vittoria Barone

Lucia Sacchetti

Affiliations

Soon will be listed here.

Abstract

We previously identified a Neisseria flavescens strain in the duodenum of celiac disease (CD) patients that induced immune inflammation in ex vivo duodenal mucosal explants and in CaCo-2 cells. We also found that vesicular trafficking was delayed after the CD-immunogenic P31-43 gliadin peptide-entered CaCo-2 cells and that Lactobacillus paracasei CBA L74 (L. paracasei-CBA) supernatant reduced peptide entry. In this study, we evaluated if metabolism and trafficking was altered in CD-N. flavescens-infected CaCo-2 cells and if any alteration could be mitigated by pretreating cells with L. paracasei-CBA supernatant, despite the presence of P31-43. We measured CaCo-2 bioenergetics by an extracellular flux analyser, N. flavescens and P31-43 intracellular trafficking by immunofluorescence, cellular stress by TBARS assay, and ATP by bioluminescence. We found that CD-N. flavescens colocalised more than control N. flavescens with early endocytic vesicles and more escaped autophagy thereby surviving longer in infected cells. P31-43 increased colocalisation of N. flavescens with early vesicles. Mitochondrial respiration was lower (P < .05) in CD-N. flavescens-infected cells versus not-treated CaCo-2 cells, whereas pretreatment with L. paracasei-CBA reduced CD-N. flavescens viability and improved cell bioenergetics and trafficking. In conclusion, CD-N. flavescens induces metabolic imbalance in CaCo-2 cells, and the L. paracasei-CBA probiotic could be used to correct CD-associated dysbiosis.

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