Glutamic-oxaloacetic Transaminase 1 Regulates Adipocyte Differentiation by Altering Nicotinamide Adenine Dinucleotide Phosphate Content

Overview

Journal Anim Biosci

Specialty Veterinary Medicine

Date 2021 Sep 3

PMID 34474530

Citations 1

Authors

Yang Yang

Zhimin Cheng

Wanfeng Zhang

Wei Hei

Chang Lu

Chunbo Cai

Yan Zhao

Pengfei Gao

Xiaohong Guo

Guoqing Cao

Bugao Li

Affiliations

Soon will be listed here.

Abstract

Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation.

Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content.

Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content.

Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.

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References

Yu X, Hiromasa Y, Tsen H, Stoops J, Roche T, Zhou Z . Structures of the human pyruvate dehydrogenase complex cores: a highly conserved catalytic center with flexible N-terminal domains. Structure. 2008; 16(1):104-14. PMC: 4807695. DOI: 10.1016/j.str.2007.10.024. View

Qing G, Li B, Vu A, Skuli N, Walton Z, Liu X . ATF4 regulates MYC-mediated neuroblastoma cell death upon glutamine deprivation. Cancer Cell. 2012; 22(5):631-44. PMC: 3510660. DOI: 10.1016/j.ccr.2012.09.021. View

Fell D, Small J . Fat synthesis in adipose tissue. An examination of stoichiometric constraints. Biochem J. 1986; 238(3):781-6. PMC: 1147204. DOI: 10.1042/bj2380781. View

Kim E, Kim W, Kang H, Kim J, Chung S, Seo Y . Acetylation of malate dehydrogenase 1 promotes adipogenic differentiation via activating its enzymatic activity. J Lipid Res. 2012; 53(9):1864-76. PMC: 3413227. DOI: 10.1194/jlr.M026567. View

Wang Y, Zhou L, Zhao Y, Wang S, Chen L, Liu L . Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress. EMBO J. 2014; 33(12):1304-20. PMC: 4194121. DOI: 10.1002/embj.201387224. View

Cho I, Park H, Ahn J, Han S, Lee J, Lim H . A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs. PLoS Genet. 2019; 15(10):e1008279. PMC: 6788688. DOI: 10.1371/journal.pgen.1008279. View

Madeira M, Costa P, Alfaia C, Lopes P, Bessa R, Lemos J . The increased intramuscular fat promoted by dietary lysine restriction in lean but not in fatty pig genotypes improves pork sensory attributes. J Anim Sci. 2013; 91(7):3177-87. DOI: 10.2527/jas.2012-5424. View

Patel K, OBrien T, Subramony S, Shuster J, Stacpoole P . The spectrum of pyruvate dehydrogenase complex deficiency: clinical, biochemical and genetic features in 371 patients. Mol Genet Metab. 2012; 106(3):385-94. PMC: 4003492. DOI: 10.1016/j.ymgme.2012.03.017. View

Gardan D, Gondret F, Louveau I . Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes. Am J Physiol Endocrinol Metab. 2006; 291(2):E372-80. DOI: 10.1152/ajpendo.00482.2005. View

10.

Alfaia C, Lopes P, Madeira M, Pestana J, Coelho D, Toldra F . Current feeding strategies to improve pork intramuscular fat content and its nutritional quality. Adv Food Nutr Res. 2019; 89:53-94. DOI: 10.1016/bs.afnr.2019.03.006. View

11.

Cong L, Ran F, Cox D, Lin S, Barretto R, Habib N . Multiplex genome engineering using CRISPR/Cas systems. Science. 2013; 339(6121):819-23. PMC: 3795411. DOI: 10.1126/science.1231143. View

12.

Gao P, Cheng Z, Li M, Zhang N, Le B, Zhang W . Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig. Asian-Australas J Anim Sci. 2019; 32(8):1084-1094. PMC: 6599955. DOI: 10.5713/ajas.18.0718. View

13.

Joo S, Kim G, Hwang Y, Ryu Y . Control of fresh meat quality through manipulation of muscle fiber characteristics. Meat Sci. 2013; 95(4):828-36. DOI: 10.1016/j.meatsci.2013.04.044. View

14.

Song T, Yang Y, Jiang S, Peng J . Novel Insights into Adipogenesis from the Perspective of Transcriptional and RNA N6-Methyladenosine-Mediated Post-Transcriptional Regulation. Adv Sci (Weinh). 2020; 7(21):2001563. PMC: 7610318. DOI: 10.1002/advs.202001563. View

15.

Listrat A, Lebret B, Louveau I, Astruc T, Bonnet M, Lefaucheur L . How Muscle Structure and Composition Influence Meat and Flesh Quality. ScientificWorldJournal. 2016; 2016:3182746. PMC: 4789028. DOI: 10.1155/2016/3182746. View

16.

Squillaro T, Peluso G, Galderisi U, Di Bernardo G . Long non-coding RNAs in regulation of adipogenesis and adipose tissue function. Elife. 2020; 9. PMC: 7392603. DOI: 10.7554/eLife.59053. View

17.

Cannata S, Engle T, Moeller S, Zerby H, Radunz A, Green M . Effect of visual marbling on sensory properties and quality traits of pork loin. Meat Sci. 2010; 85(3):428-34. DOI: 10.1016/j.meatsci.2010.02.011. View

18.

Zhou X, Liu Y, Zhang L, Kong X, Li F . Serine-to-glycine ratios in low-protein diets regulate intramuscular fat by affecting lipid metabolism and myofiber type transition in the skeletal muscle of growing-finishing pigs. Anim Nutr. 2021; 7(2):384-392. PMC: 8245814. DOI: 10.1016/j.aninu.2020.08.011. View

19.

Hocquette J, Gondret F, Baeza E, Medale F, Jurie C, Pethick D . Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers. Animal. 2012; 4(2):303-19. DOI: 10.1017/S1751731109991091. View

20.

Spurlock M, Gabler N . The development of porcine models of obesity and the metabolic syndrome. J Nutr. 2008; 138(2):397-402. DOI: 10.1093/jn/138.2.397. View