Upregulated LncRNA Gm2044 Inhibits Male Germ Cell Development by Acting As miR-202 Host Gene

Overview

Journal Anim Cells Syst (Seoul)

Specialty Biology

Date 2019 Apr 6

PMID 30949400

Citations 15

Authors

Meng Liang

Ke Hu

Chaofan He

Jinzhao Zhou

Yaping Liao

Affiliations

Soon will be listed here.

Abstract

Long non-coding RNAs (lncRNAs) have been found to participate in the regulation of human spermatogenic cell development. However, little is known about the abnormal expression of lncRNAs associated with spermatogenic failure and their molecular mechanisms. Using lncRNA microarray of testicular tissue for male infertility and bioinformatics methods, we identified the relatively conserved lncRNA Gm2044 which may play important roles in non-obstructive azoospermia. The UCSC Genome Browser showed that lncRNA Gm2044 is the miR-202 host gene. This study revealed that lncRNA Gm2044 and miR-202 were significantly increased in non-obstructive azoospermia of spermatogonial arrest. The mRNA and protein levels of , a known direct target gene of miR-202, were regulated by lncRNA Gm2044. Furthermore, the miR-202- signalling pathway was shown to mediate the suppressive effects of lncRNA Gm2044 on the proliferation of human testicular embryonic carcinoma cells. Understanding of the molecular signalling pathways for lncRNA-regulated spermatogenesis will provide new clues into the pathogenesis and treatment of patients with male infertility.

Citing Articles

Mechanism of LncRNA Gm2044 in germ cell development.

Zhu Q, Sun J, An C, Li X, Xu S, He Y Front Cell Dev Biol. 2024; 12:1410914.

PMID: 39027044 PMC: 11255455. DOI: 10.3389/fcell.2024.1410914.

Dysregulated expression of IGSF11-AS1 and BVES-AS in azoospermia and its correlation with serum hormone levels.

Ibrahim Jebur M, Kadhim Muttwaqi N, Saleh Saleh A, Safaralizadeh R Biomark Med. 2024; 18(9):485-492.

PMID: 38881522 PMC: 11285263. DOI: 10.1080/17520363.2024.2345581.

The lncRNA Gm8097 is associated with hypospermatogenesis.

Lei B, Ye L, Qiu Z, Zhang S Clin Exp Reprod Med. 2024; 51(4):314-323.

PMID: 38853128 PMC: 11617910. DOI: 10.5653/cerm.2024.06835.

DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression.

Hua R, Chu Q, Guo F, Chen Q, Li M, Zhou X Anal Cell Pathol (Amst). 2023; 2023:1477658.

PMID: 38152068 PMC: 10752680. DOI: 10.1155/2023/1477658.

Interaction of lncRNA Gm2044 and EEF2 promotes estradiol synthesis in ovarian follicular granulosa cells.

Hu K, Wang C, Xu Y, Li F, Han X, Song C J Ovarian Res. 2023; 16(1):171.

PMID: 37612724 PMC: 10464411. DOI: 10.1186/s13048-023-01232-z.

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