Genetic Variations in E6, E7 and the Long Control Region of Human Papillomavirus Type 16 Among Patients with Cervical Lesions in Xinjiang, China

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2019 Apr 2

PMID 30930693

Citations 19

Authors

Xiangyi Zhe

Huizhen Xin

Zhenzhen Pan

Fuyuan Jin

Weinan Zheng

Hongtao Li

Dongmei Li

Dongdong Cao

Ying Li

Chunhe Zhang

Shaowei Fu

Renfu Shao

Zemin Pan

Affiliations

Soon will be listed here.

Abstract

Background: Xinjiang is one of the areas with the highest incidence of cervical cancer in China. Genetic variation in Human papillomavirus type 16 (HPV16) may increase the ability of the virus to mediate carcinogenesis and immune escape, which are risk factors for the progression of cervical cancer. We investigated polymorphism in HPV16 and the distribution of its sub-lineages in the region by analyzing the , and () gene sequences from women with HPV16-positive cervical samples in Xinjiang.

Methods: A total of 138 cases of cervical lesions and squamous cell carcinoma with infection of HPV16 virus were collected. The and genes and of HPV16 virus were sequenced and compared with the HPV16 European prototype reference and other HPV16 mutants for single nucleotide polymorphisms. Neighbor-joining phylogenetic trees were constructed using E6, E7 and LCR sequences.

Results: Fourteen missense mutations were found in the gene; the loci with the highest mutation frequency were T350G (36/75, 48%) and T178G (19/75, 25.3%). In the gene, the locus with the highest mutation frequency was A647G (18/75, 24%). A total of 33 polymorphic sites were found in the , of which T7447C (39/95, 40.1%) was the most frequent.

Conclusion: HPV16 in Xinjiang is mainly of the European variant, followed by the Asian variant type; no Africa 1, 2 or Asia-America variant types were found.

Citing Articles

Identification of HPV16 Lineages in South African and Mozambican Women with Normal and Abnormal Cervical Cytology.

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Analysis of human papillomavirus type 16 E4, E5 and L2 gene variations among women with cervical infection in Xinjiang, China.

Cheng H, Dong Y, Wang L, Zhao X, Zhe X, Li D BMC Med Genomics. 2024; 17(1):179.

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The analysis of HPV integration sites based on nanopore sequencing and the profiling changes along the course of photodynamic therapy.

Li X, Wei X, Liu X, Wang N, Xu F, Liu X BMC Cancer. 2023; 23(1):1052.

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Genetic variation of E6 and E7 genes of human papillomavirus type 16 from central China.

Li T, Yang Z, Zhang C, Wang S, Mei B Virol J. 2023; 20(1):217.

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