Analysis of Genetic Variation in Human Papillomavirus Type 16 E1 and E2 in Women with Cervical Infection in Xinjiang, China

Overview

Journal BMC Med Genomics

Publisher Biomed Central

Specialty Genetics

Date 2021 Nov 13

PMID 34772425

Citations 4

Authors

Luyue Wang

Fang Wang

Shaowei Fu

Chunhe Zhang

Xiangyi Zhe

Hongtao Li

Dongmei Li

Renfu Shao

Zemin Pan

Affiliations

Soon will be listed here.

Abstract

Background: Xinjiang is one of the regions with a high incidence of cervical cancer, and the genetic variation of human papillomavirus may increase its ability to infect the human body and enhance virus-mediated immune escape ability.

Methods: Sanger sequencing of the HPV16 genome from 165 samples positive for HPV16 infection and phylogenetic analysis of the E1 and E2 genes revealed the gene polymorphism of HPV16 in Xinjiang.

Results: The results showed that there were 109 samples with variations in HPV16 E1, 48 sites with nucleotide variations (19 missense variations and 29 synonymous variations), and 91 samples with variations in HPV16 E2, 25 sites with nucleotide variations (20 missense variations and five synonymous variations).

Conclusions: From the phylogenetic tree results, 149 samples were of the European variant and 16 samples were of the Asian variant. No African or North American/Asian variant types were found.

Citing Articles

Identification of HPV16 Lineages in South African and Mozambican Women with Normal and Abnormal Cervical Cytology.

Maueia C, Carulei O, Murahwa A, Taku O, Manjate A, Mussa T Viruses. 2024; 16(8).

PMID: 39205288 PMC: 11360388. DOI: 10.3390/v16081314.

Analysis of human papillomavirus type 16 E4, E5 and L2 gene variations among women with cervical infection in Xinjiang, China.

Cheng H, Dong Y, Wang L, Zhao X, Zhe X, Li D BMC Med Genomics. 2024; 17(1):179.

PMID: 38965538 PMC: 11225290. DOI: 10.1186/s12920-024-01926-3.

Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice: Sublineage A1 Drives Multi-Organ Carcinogenesis.

Cochicho D, Nunes A, Gomes J, Martins L, Cunha M, Medeiros-Fonseca B Int J Mol Sci. 2022; 23(20).

PMID: 36293226 PMC: 9604181. DOI: 10.3390/ijms232012371.

Synthesis and Anticancer Activity of Rhopaladins' Analog RPDPD Against the HeLa Human Cervical Cancer Cell Line.

Chen F, Wang H, Kong L, Chen Q, Ke L, Dai H Front Chem. 2022; 10:921276.

PMID: 35844635 PMC: 9277706. DOI: 10.3389/fchem.2022.921276.

References

Lusky M, Hurwitz J, Seo Y . Cooperative assembly of the bovine papilloma virus E1 and E2 proteins on the replication origin requires an intact E2 binding site. J Biol Chem. 1993; 268(21):15795-803. View

Melendy T, Sedman J, Stenlund A . Cellular factors required for papillomavirus DNA replication. J Virol. 1995; 69(12):7857-67. PMC: 189730. DOI: 10.1128/JVI.69.12.7857-7867.1995. View

Amaro-Filho S, Chaves C, Felix S, Basto D, de Almeida L, Moreira M . HPV DNA methylation at the early promoter and E1/E2 integrity: A comparison between HPV16, HPV18 and HPV45 in cervical cancer. Papillomavirus Res. 2018; 5:172-179. PMC: 6046686. DOI: 10.1016/j.pvr.2018.04.002. View

Zhe X, Xin H, Pan Z, Jin F, Zheng W, Li H . Genetic variations in E6, E7 and the long control region of human papillomavirus type 16 among patients with cervical lesions in Xinjiang, China. Cancer Cell Int. 2019; 19:65. PMC: 6425590. DOI: 10.1186/s12935-019-0774-5. View

Dai S, Yao Y, Yan Z, Zhou Z, Shi L, Wang X . The association of human papillomavirus type 16 E2 variations with cervical cancer in a Han Chinese population. Infect Genet Evol. 2018; 64:241-248. DOI: 10.1016/j.meegid.2018.07.002. View

Arbyn M, Weiderpass E, Bruni L, de Sanjose S, Saraiya M, Ferlay J . Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis. Lancet Glob Health. 2019; 8(2):e191-e203. PMC: 7025157. DOI: 10.1016/S2214-109X(19)30482-6. View

Pan Z, Song Y, Zhe X, Wang W, Zhu J, Zheng W . Screening for HPV infection in exfoliated cervical cells of women from different ethnic groups in Yili, Xinjiang, China. Sci Rep. 2019; 9(1):3468. PMC: 6400895. DOI: 10.1038/s41598-019-39790-2. View

Filho S, Bertoni N, Castro Brant A, Vidal J, Felix S, Cavalcanti S . Methylation at 3'LCR of HPV16 can be affected by patient age and disruption of E1 or E2 genes. Virus Res. 2017; 232:48-53. DOI: 10.1016/j.virusres.2017.01.022. View

Tsakogiannis D, Ruether I, Kyriakopoulou Z, Pliaka V, Theoharopoulou A, Skordas V . Sequence variation analysis of the E2 gene of human papilloma virus type 16 in cervical lesions from women in Greece. Arch Virol. 2012; 157(5):825-32. DOI: 10.1007/s00705-012-1236-8. View

10.

Fradet-Turcotte A, Bergeron-Labrecque F, Moody C, Lehoux M, Laimins L, Archambault J . Nuclear accumulation of the papillomavirus E1 helicase blocks S-phase progression and triggers an ATM-dependent DNA damage response. J Virol. 2011; 85(17):8996-9012. PMC: 3165840. DOI: 10.1128/JVI.00542-11. View

11.

Yao Y, Yan Z, Dai S, Li C, Yang L, Liu S . Human Papillomavirus Type 16 Mutations Associated with Cervical Cancer in a Han Chinese Population. Int J Med Sci. 2019; 16(7):1042-1049. PMC: 6643129. DOI: 10.7150/ijms.34279. View

12.

McBride A, Romanczuk H, Howley P . The papillomavirus E2 regulatory proteins. J Biol Chem. 1991; 266(28):18411-4. View

13.

Brant A, Menezes A, Felix S, Almeida L, Moreira M . Preferential expression of a HPV genotype in invasive cervical carcinomas infected by multiple genotypes. Genomics. 2020; 112(5):2942-2948. DOI: 10.1016/j.ygeno.2020.05.009. View

14.

Sabol I, Matovina M, Milutin Gasperov N, Grce M . Identification of a novel human papillomavirus type 16 E1 gene variant with potentially reduced oncogenicity. J Med Virol. 2008; 80(12):2134-40. DOI: 10.1002/jmv.21304. View

15.

Evans M, James C, Bristol M, Nulton T, Wang X, Kaur N . Human Papillomavirus 16 E2 Regulates Keratinocyte Gene Expression Relevant to Cancer and the Viral Life Cycle. J Virol. 2018; 93(4). PMC: 6364038. DOI: 10.1128/JVI.01941-18. View

16.

Seedorf K, Oltersdorf T, Krammer G, Rowekamp W . Identification of early proteins of the human papilloma viruses type 16 (HPV 16) and type 18 (HPV 18) in cervical carcinoma cells. EMBO J. 1987; 6(1):139-44. PMC: 553369. DOI: 10.1002/j.1460-2075.1987.tb04731.x. View

17.

Wang J, Tang D, Wang K, Wang J, Zhang Z, Chen Y . HPV genotype prevalence and distribution during 2009-2018 in Xinjiang, China: baseline surveys prior to mass HPV vaccination. BMC Womens Health. 2019; 19(1):90. PMC: 6615222. DOI: 10.1186/s12905-019-0785-3. View

18.

Di J, Rutherford S, Chu C . Review of the Cervical Cancer Burden and Population-Based Cervical Cancer Screening in China. Asian Pac J Cancer Prev. 2015; 16(17):7401-7. DOI: 10.7314/apjcp.2015.16.17.7401. View

19.

McBride A . The papillomavirus E2 proteins. Virology. 2013; 445(1-2):57-79. PMC: 3783563. DOI: 10.1016/j.virol.2013.06.006. View

20.

Bristol M, Wang X, Smith N, Son M, Evans M, Morgan I . DNA Damage Reduces the Quality, but Not the Quantity of Human Papillomavirus 16 E1 and E2 DNA Replication. Viruses. 2016; 8(6). PMC: 4926195. DOI: 10.3390/v8060175. View