Characteristics of Intestinal Microecology During Mesenchymal Stem Cell-Based Therapy for Mouse Acute Liver Injury

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2019 Mar 15

PMID 30867666

Citations 13

Authors

Xiaotian Dong

Xudong Feng

Jingqi Liu

Yanping Xu

Qiaoling Pan

Zongxin Ling

Jiong Yu

Jinfeng Yang

Lanjuan Li

Hongcui Cao

Affiliations

Soon will be listed here.

Abstract

Background: The mechanisms of mesenchymal stem cell (MSC) transplantation to protect against acute liver injury have been well studied within the liver. However, the associated changes in the intestinal microbiota during this process are poorly understood.

Methods: In this study, compact bone-derived MSCs were injected into mice after carbon tetrachloride (CCl) administration. Potential curative effect of MSC was evaluated by survival rate and biochemical and pathological results. Overall structural changes of microbial communities and alterations in the intestinal microbiota were assessed by sequenced 16S rRNA amplicon libraries from the contents of the cecum and colon.

Results: MSCs significantly reduced the serum levels of aspartate transaminase and alanine transaminase and improved the histopathology and survival rate. Lower expression and discontinuous staining of zonula occludens, as well as disrupted tight junctions, were observed in CCl-treated mice at 48 h compared with MSC-transplanted mice. Moreover, MSC transplantation to the liver leads to intestinal microbiota changes that were reflected in the decreased abundance of Bacteroidetes and and increased abundance of Firmicutes , , and at the initial time point compared with that in CCl-treated mice. In addition, phylogenetic investigation of communities by the reconstruction of unobserved states (PICRUSt) based on the Greengenes database revealed functional biomarkers of MSC-transplanted mice involved in cell motility, signal transduction, membrane transport, transcription, and metabolism of lipids, cofactors, vitamins, terpenoids, and polyketides, as well as xenobiotics.

Conclusion: The initial alterations in the Firmicutes/Bacteroidetes ratio, which resulted from MSC infusion to the liver, maintain intestinal mucosal biology and homeostasis that may be beneficial to liver repair.

Citing Articles

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Human umbilical cord mesenchymal stem cells regulate immunoglobulin a secretion and remodel the diversification of intestinal microbiota to improve colitis.

Liu A, Wang X, Liang X, Wang W, Li C, Qian J Front Cell Infect Microbiol. 2022; 12:960208.

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Human umbilical cord-derived mesenchymal stem cells ameliorate experimental colitis by normalizing the gut microbiota.

Yang F, Ni B, Liu Q, He F, Li L, Zhong X Stem Cell Res Ther. 2022; 13(1):475.

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Characterising the Intestinal Bacterial and Fungal Microbiome Associated With Different Cytokine Profiles in Two Pre-Treated Rats With D-Galactosamine-Induced Liver Injury.

Zha H, Li Q, Chang K, Xia J, Li S, Tang R Front Immunol. 2022; 13:791152.

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hUC-MSCs: evaluation of acute and long-term routine toxicity testing in mice and rats.

Xu J, Liu G, Wang X, Hu Y, Luo H, Ye L Cytotechnology. 2022; 74(1):17-29.

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