Obesity Alters Gut Microbial Ecology

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2005 Jul 22

PMID 16033867

Citations 2650

Authors

Ruth E Ley

Fredrik Backhed

Peter Turnbaugh

Catherine A Lozupone

Robin D Knight

Jeffrey I Gordon

Affiliations

Soon will be listed here.

Abstract

We have analyzed 5,088 bacterial 16S rRNA gene sequences from the distal intestinal (cecal) microbiota of genetically obese ob/ob mice, lean ob/+ and wild-type siblings, and their ob/+ mothers, all fed the same polysaccharide-rich diet. Although the majority of mouse gut species are unique, the mouse and human microbiota(s) are similar at the division (superkingdom) level, with Firmicutes and Bacteroidetes dominating. Microbial-community composition is inherited from mothers. However, compared with lean mice and regardless of kinship, ob/ob animals have a 50% reduction in the abundance of Bacteroidetes and a proportional increase in Firmicutes. These changes, which are division-wide, indicate that, in this model, obesity affects the diversity of the gut microbiota and suggest that intentional manipulation of community structure may be useful for regulating energy balance in obese individuals. The sequences reported in this paper have been deposited in the GenBank database [accession nos. DQ 014552--DQ 015671 (mothers) and AY 989911--AY 993908 (offspring)].

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References

Papineau D, Walker J, Mojzsis S, Pace N . Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia. Appl Environ Microbiol. 2005; 71(8):4822-32. PMC: 1183352. DOI: 10.1128/AEM.71.8.4822-4832.2005. View

Hugenholtz P . Exploring prokaryotic diversity in the genomic era. Genome Biol. 2002; 3(2):REVIEWS0003. PMC: 139013. DOI: 10.1186/gb-2002-3-2-reviews0003. View

Sonnenburg J, Xu J, Leip D, Chen C, Westover B, Weatherford J . Glycan foraging in vivo by an intestine-adapted bacterial symbiont. Science. 2005; 307(5717):1955-9. DOI: 10.1126/science.1109051. View

Harris J, Kelley S, Pace N . New perspective on uncultured bacterial phylogenetic division OP11. Appl Environ Microbiol. 2004; 70(2):845-9. PMC: 348892. DOI: 10.1128/AEM.70.2.845-849.2004. View

Suzuki K, Meek B, Doi Y, Muramatsu M, Chiba T, Honjo T . Aberrant expansion of segmented filamentous bacteria in IgA-deficient gut. Proc Natl Acad Sci U S A. 2004; 101(7):1981-6. PMC: 357038. DOI: 10.1073/pnas.0307317101. View

Backhed F, Ley R, Sonnenburg J, Peterson D, Gordon J . Host-bacterial mutualism in the human intestine. Science. 2005; 307(5717):1915-20. DOI: 10.1126/science.1104816. View

Posada D, Crandall K . MODELTEST: testing the model of DNA substitution. Bioinformatics. 1999; 14(9):817-8. DOI: 10.1093/bioinformatics/14.9.817. View

Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman J . Positional cloning of the mouse obese gene and its human homologue. Nature. 1994; 372(6505):425-32. DOI: 10.1038/372425a0. View

Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389-402. PMC: 146917. DOI: 10.1093/nar/25.17.3389. View

10.

Warwick B, Romsos D . Energy balance in adrenalectomized ob/ob mice: effects of dietary starch and glucose. Am J Physiol. 1988; 255(1 Pt 2):R141-8. DOI: 10.1152/ajpregu.1988.255.1.R141. View

11.

Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar . ARB: a software environment for sequence data. Nucleic Acids Res. 2004; 32(4):1363-71. PMC: 390282. DOI: 10.1093/nar/gkh293. View

12.

Backhed F, Ding H, Wang T, Hooper L, Koh G, Nagy A . The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci U S A. 2004; 101(44):15718-23. PMC: 524219. DOI: 10.1073/pnas.0407076101. View

13.

Xu J, Bjursell M, Himrod J, Deng S, Carmichael L, Chiang H . A genomic view of the human-Bacteroides thetaiotaomicron symbiosis. Science. 2003; 299(5615):2074-6. DOI: 10.1126/science.1080029. View

14.

Ferraris R, Vinnakota R . Intestinal nutrient transport in genetically obese mice. Am J Clin Nutr. 1995; 62(3):540-6. DOI: 10.1093/ajcn/62.3.540. View

15.

Tringe S, von Mering C, Kobayashi A, Salamov A, Chen K, Chang H . Comparative metagenomics of microbial communities. Science. 2005; 308(5721):554-7. DOI: 10.1126/science.1107851. View

16.

Cole J, Chai B, Farris R, Wang Q, Kulam S, McGarrell D . The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res. 2004; 33(Database issue):D294-6. PMC: 539992. DOI: 10.1093/nar/gki038. View

17.

Ronquist F, Huelsenbeck J . MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003; 19(12):1572-4. DOI: 10.1093/bioinformatics/btg180. View

18.

GUSTAFSON R, Bowen R . Antibiotic use in animal agriculture. J Appl Microbiol. 1998; 83(5):531-41. DOI: 10.1046/j.1365-2672.1997.00280.x. View

19.

Handelsman J, Rondon M, Brady S, Clardy J, Goodman R . Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol. 1998; 5(10):R245-9. DOI: 10.1016/s1074-5521(98)90108-9. View

20.

Stamatakis A, Ludwig T, Meier H . RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees. Bioinformatics. 2004; 21(4):456-63. DOI: 10.1093/bioinformatics/bti191. View