Purification to Homogeneity of B Cell Stimulating Factor. A Molecule That Stimulates Proliferation of Multiple Lymphokine-dependent Cell Lines

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1986 Jun 1

PMID 3086481

Citations 45

Authors

K Grabstein

J Eisenman

D Mochizuki

K Shanebeck

P Conlon

T Hopp

C March

S Gillis

Affiliations

Soon will be listed here.

Abstract

Murine B cell stimulating factor 1 (BSF-1) was purified to homogeneity from supernatants of a stimulated thymoma cell line. A protein of 18.4 kD with a unique N-terminal amino acid sequence was identified. BSF-1 had a sp act of at least 3.28 X 10(8) U/mg. In addition to its B cell-stimulatory activity, BSF-1 also stimulated the proliferation of several IL-2- and IL-3-dependent cell lines. We conclude that BSF-1 is both a growth factor and a differentiation factor. Finally, these results also suggest additional biologic properties of BSF-1 on lineages besides B lymphocytes.

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