Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle

Overview

Journal Front Bioeng Biotechnol

Date 2019 Mar 7

PMID 30838203

Citations 21

Authors

Andrew J Capel

Rowan P Rimington

Jacob W Fleming

Darren J Player

Luke A Baker

Mark C Turner

Julia M Jones

Neil R W Martin

Richard A Ferguson

Vivek C Mudera

Mark P Lewis

Affiliations

Soon will be listed here.

Abstract

Tissue engineered skeletal muscle allows investigation of the cellular and molecular mechanisms that regulate skeletal muscle pathology. The fabricated model must resemble characteristics of tissue and incorporate cost-effective and high content primary human tissue. Current models are limited by low throughput due to the complexities associated with recruiting tissue donors, donor specific variations, as well as cellular senescence associated with passaging. This research presents a method using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate reproducible and scalable tissue engineered primary human muscle, possessing aligned mature myotubes reminiscent of tissue. Many existing models are bespoke causing variability when translated between laboratories. To this end, a scalable model has been developed (25-500 μL construct volumes) allowing fabrication of mature primary human skeletal muscle. This research provides a strategy to overcome limited biopsy cell numbers, enabling high throughput screening of functional human tissue.

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