Ex Vivo Real-time Monitoring of Volatile Metabolites Resulting from Nasal Odorant Metabolism

Overview

Journal Sci Rep

Specialty Science

Date 2019 Feb 23

PMID 30792537

Citations 8

Authors

Aline Robert-Hazotte

Rachel Schoumacker

Etienne Semon

Loic Briand

Elisabeth Guichard

Jean-Luc Le Quere

Philippe Faure

Jean-Marie Heydel

Affiliations

Soon will be listed here.

Abstract

Odorant-metabolizing enzymes are critically involved in the clearance of odorant molecules from the environment of the nasal neuro-olfactory tissue to maintain the sensitivity of olfactory detection. Odorant metabolism may also generate metabolites in situ, the characterization and function of which in olfaction remain largely unknown. Here, we engineered and validated an ex vivo method to measure odorant metabolism in real-time. Glassware containing an explant of rat olfactory mucosa was continuously flushed with an odorant flow and was coupled to a proton transfer reaction-mass spectrometer for volatile compound analysis. Focusing on carboxylic esters and diketone odorants, we recorded the metabolic uptake of odorants by the mucosa, concomitantly with the release of volatile odorant metabolites in the headspace. These results significantly change the picture of real-time in situ odorant metabolism and represent a new step forward in the investigation of the function of odorant metabolites in the peripheral olfactory process. Our method allows the systematic identification of odorant metabolites using a validated animal model and permits the screening of olfactory endogenously produced chemosensory molecules.

Citing Articles

Functions of human olfactory mucus and age-dependent changes.

Shirai T, Takase D, Yokoyama J, Nakanishi K, Uehara C, Saito N Sci Rep. 2023; 13(1):971.

PMID: 36653421 PMC: 9846672. DOI: 10.1038/s41598-023-27937-1.

Odorant Metabolism in Humans.

Kornbausch N, Debong M, Buettner A, Heydel J, Loos H Angew Chem Int Ed Engl. 2022; 61(35):e202202866.

PMID: 35522818 PMC: 9541901. DOI: 10.1002/anie.202202866.

The odorant metabolizing enzyme UGT2A1: Immunolocalization and impact of the modulation of its activity on the olfactory response.

Neiers F, Jarriault D, Menetrier F, Faure P, Briand L, Heydel J PLoS One. 2021; 16(3):e0249029.

PMID: 33765098 PMC: 7993815. DOI: 10.1371/journal.pone.0249029.

Odor coding in the mammalian olfactory epithelium.

Kurian S, Naressi R, Manoel D, Barwich A, Malnic B, Saraiva L Cell Tissue Res. 2021; 383(1):445-456.

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Sensory Perception of Non-Deuterated and Deuterated Organic Compounds.

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