Use of Anti-idiotypic Antibodies As Cell-surface Receptor Probes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1978 May 1

PMID 307766

Citations 74

Authors

K Sege

P A Peterson

Affiliations

Soon will be listed here.

Abstract

Anti-idiotypic antibodies have been raised against antibodies to retinol-binding protein (RBP) and to insulin. After absorption the anti-idiotypic antibodies recognized the antigen-combining sites of the antibodies used as the immunogen but of no other antibodies. Some of the anti-idiotypic antibodies raised against antibodies to RBP bound specifically to rat intestine epithelial cells, which have a physiological cell-surface receptor for RBP. The RBP receptor mediates the uptake of retinol from RBP to the cells. This uptake was abolished in a concentration-dependent manner by the anti-idiotypic antibodies, which obviously competed with RBP for binding to the receptor.Anti-idiotypic antibodies against antibodies to insulin inhibited the binding of (125)I-labeled insulin to isolated rat epididymal fat cells, whereas anti-idiotypic antibodies raised against antibodies to RBP had no effect. Furthermore, on interacting with young rat thymocytes, anti-idiotypic antibodies against antibodies to insulin stimulated the uptake by the cells of alpha-aminoisobutyric acid, thereby mimicking the effect of insulin. These results suggest that in some cases anti-idiotypic antibodies may be useful tools in elucidating structure-function relationships for cell-membrane receptors.

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References

GREENWOOD F, Hunter W, GLOVER J . THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963; 89:114-23. PMC: 1202279. DOI: 10.1042/bj0890114. View

Sege K, Peterson P . Anti-idiotypic antibodies against anti-vitamin A transporting protein react with prealbumin. Nature. 1978; 271(5641):167-8. DOI: 10.1038/271167a0. View

Rowley D, Kohler H, Schreiber H, Kaye S, Lorbach I . Suppression by autogenous complementary idiotypes: the priority of the first response. J Exp Med. 1976; 144(4):946-59. PMC: 2190429. DOI: 10.1084/jem.144.4.946. View

Rask L, Peterson P . In vitro uptake of vitamin A from the retinol-binding plasma protein to mucosal epithelial cells from the monkey's small intestine. J Biol Chem. 1976; 251(20):6360-6. View

Ostberg L, Sege K, Rask L, Peterson P . Isolation of radiolabelled H-2 antigens [proceedings]. Folia Biol (Praha). 1976; 22(6):372-3. View

Ruoslahti E, Wigzell H . Breakage of tolerance to alpha foetoprotein in monkeys. Nature. 1975; 255(5511):716-7. DOI: 10.1038/255716a0. View

Peterson P, Evrin P, BERGGARD I . Differentiation of glomerular, tubular, and normal proteinuria: determinations of urinary excretion of beta-2-macroglobulin, albumin, and total protein. J Clin Invest. 1969; 48(7):1189-98. PMC: 322340. DOI: 10.1172/JCI106083. View

Dorval G, Welsh K, Wigzell H . Labeled staphylococcal protein A as an immunological probe in the analysis of cell surface markers. Scand J Immunol. 1974; 3(4):405-11. DOI: 10.1111/j.1365-3083.1974.tb01273.x. View

Peterson P, Rask L, Lindblom J . Highly purified papain-solubilized HL-A antigens contain beta2-microglobulin. Proc Natl Acad Sci U S A. 1974; 71(1):35-9. PMC: 387926. DOI: 10.1073/pnas.71.1.35. View

10.

Goldfine I, Gardner J, Neville Jr D . Insulin action in isolated rat thymocytes. I. Binding of 125 I-insulin and stimulation of -aminoisobutyric acid transport. J Biol Chem. 1972; 247(21):6919-26. View

11.

Freychet P, Roth J, Neville Jr D . Insulin receptors in the liver: specific binding of ( 125 I)insulin to the plasma membrane and its relation to insulin bioactivity. Proc Natl Acad Sci U S A. 1971; 68(8):1833-7. PMC: 389303. DOI: 10.1073/pnas.68.8.1833. View

12.

Peterson P, Nilsson S, Ostberg L, Rask L, Vahlquist A . Aspects of the metabolism of retinol-binding protein and retinol. Vitam Horm. 1974; 32:181-214. DOI: 10.1016/s0083-6729(08)60012-6. View

13.

Vahlquist A, Peterson P . Combination of specific antibodies with the human vitamin A-transporting protein complex. J Biol Chem. 1973; 248(11):4040-6. View

14.

Hjelm H, Hjelm K, Sjoquist J . Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett. 1972; 28(1):73-6. DOI: 10.1016/0014-5793(72)80680-x. View

15.

Kahn C . Membrane receptors for hormones and neurotransmitters. J Cell Biol. 1976; 70(2 pt 1):261-86. PMC: 2109819. DOI: 10.1083/jcb.70.2.261. View

16.

Flier J, Kahn C, Roth J, Bar R . Antibodies that impair insulin receptor binding in an unusual diabetic syndrome with severe insulin resistance. Science. 1975; 190(4209):63-5. DOI: 10.1126/science.170678. View

17.

Cuatrecasas P . Insulin--receptor interactions in adipose tissue cells: direct measurement and properties. Proc Natl Acad Sci U S A. 1971; 68(6):1264-8. PMC: 389168. DOI: 10.1073/pnas.68.6.1264. View

18.

Peterson P . Characteristics of a vitamin A-transporting protein complex occurring in human serum. J Biol Chem. 1971; 246(1):34-43. View

19.

GLIEMANN J . Assay of insulin-like activity by the isolated fat cell method. I. Factors influencing the response to crystalline insulin. Diabetologia. 1967; 3(4):382-8. DOI: 10.1007/BF02342631. View