MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection

Overview

Journal Sci Rep

Specialty Science

Date 2016 Apr 27

PMID 27112594

Citations 56

Authors

Jiming Gao

Shuqi Xiao

Yihong Xiao

Xiangpeng Wang

Chong Zhang

Qin Zhao

Yuchen Nan

Baicheng Huang

Hongliang Liu

Ningning Liu

Junhua Lv

Taofeng Du

Yani Sun

Yang Mu

Gang Wang

Shahid Faraz Syed

Gaiping Zhang

Julian A Hiscox

Ian Goodfellow

En-Min Zhou

Affiliations

Soon will be listed here.

Abstract

Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) is an important swine disease worldwide. PRRSV has a limited tropism for certain cells, which may at least in part be attributed to the expression of the necessary cellular molecules serving as the virus receptors or factors on host cells for virus binding or entry. However, these molecules conferring PRRSV infection have not been fully characterized. Here we show the identification of non-muscle myosin heavy chain 9 (MYH9) as an essential factor for PRRSV infection using the anti-idiotypic antibody specific to the PRRSV glycoprotein GP5. MYH9 physically interacts with the PRRSV GP5 protein via its C-terminal domain and confers susceptibility of cells to PRRSV infection. These findings indicate that MYH9 is an essential factor for PRRSV infection and provide new insights into PRRSV-host interactions and viral entry, potentially facilitating development of control strategies for this important swine disease.

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References

Zhou Y, Hao X, Tian Z, Tong G, Yoo D, An T . Highly virulent porcine reproductive and respiratory syndrome virus emerged in China. Transbound Emerg Dis. 2008; 55(3-4):152-64. DOI: 10.1111/j.1865-1682.2008.01020.x. View

Zhou E, Xiao Y, Shi Y, Li X, Ma L, Jing S . Generation of internal image monoclonal anti-idiotypic antibodies against idiotypic antibodies to GP5 antigen of porcine reproductive and respiratory syndrome virus. J Virol Methods. 2008; 149(2):300-8. DOI: 10.1016/j.jviromet.2008.01.010. View

Vicente-Manzanares M, Ma X, Adelstein R, Horwitz A . Non-muscle myosin II takes centre stage in cell adhesion and migration. Nat Rev Mol Cell Biol. 2009; 10(11):778-90. PMC: 2834236. DOI: 10.1038/nrm2786. View

Van Gorp H, Van Breedam W, Delputte P, Nauwynck H . The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection. Arch Virol. 2009; 154(12):1939-43. DOI: 10.1007/s00705-009-0527-1. View

Das P, Dinh P, Ansari I, de Lima M, Osorio F, Pattnaik A . The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163. J Virol. 2009; 84(4):1731-40. PMC: 2812361. DOI: 10.1128/JVI.01774-09. View

Van Gorp H, Van Breedam W, Van Doorsselaere J, Delputte P, Nauwynck H . Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus. J Virol. 2009; 84(6):3101-5. PMC: 2826032. DOI: 10.1128/JVI.02093-09. View

Arii J, Goto H, Suenaga T, Oyama M, Kozuka-Hata H, Imai T . Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1. Nature. 2010; 467(7317):859-62. DOI: 10.1038/nature09420. View

Johnson C, Griggs T, Gnanandarajah J, Murtaugh M . Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses. J Gen Virol. 2011; 92(Pt 5):1107-1116. PMC: 3139420. DOI: 10.1099/vir.0.030213-0. View

Hanisch J, Kolm R, Wozniczka M, Bumann D, Rottner K, Stradal T . Activation of a RhoA/myosin II-dependent but Arp2/3 complex-independent pathway facilitates Salmonella invasion. Cell Host Microbe. 2011; 9(4):273-85. DOI: 10.1016/j.chom.2011.03.009. View

10.

Wang C, Zhao Q, Liang C, Dang L, Ma Y, Gao J . Complete genome sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus variant. J Virol. 2012; 86(16):8906. PMC: 3421716. DOI: 10.1128/JVI.01281-12. View

11.

Prather R, Rowland R, Ewen C, Trible B, Kerrigan M, Bawa B . An intact sialoadhesin (Sn/SIGLEC1/CD169) is not required for attachment/internalization of the porcine reproductive and respiratory syndrome virus. J Virol. 2013; 87(17):9538-46. PMC: 3754101. DOI: 10.1128/JVI.00177-13. View

12.

Wang X, Wei R, Li Q, Liu H, Huang B, Gao J . PK-15 cells transfected with porcine CD163 by PiggyBac transposon system are susceptible to porcine reproductive and respiratory syndrome virus. J Virol Methods. 2013; 193(2):383-90. DOI: 10.1016/j.jviromet.2013.06.035. View

13.

Sun Y, Qi Y, Liu C, Gao W, Chen P, Fu L . Nonmuscle myosin heavy chain IIA is a critical factor contributing to the efficiency of early infection of severe fever with thrombocytopenia syndrome virus. J Virol. 2013; 88(1):237-48. PMC: 3911693. DOI: 10.1128/JVI.02141-13. View

14.

Wang C, Huang B, Kong N, Li Q, Ma Y, Li Z . A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit. Vet Res. 2013; 44:104. PMC: 4176086. DOI: 10.1186/1297-9716-44-104. View

15.

Gao J, Ji P, Zhang M, Wang X, Li N, Wang C . GP5 expression in Marc-145 cells inhibits porcine reproductive and respiratory syndrome virus infection by inducing beta interferon activity. Vet Microbiol. 2014; 174(3-4):409-418. DOI: 10.1016/j.vetmic.2014.09.030. View

16.

Arii J, Hirohata Y, Kato A, Kawaguchi Y . Nonmuscle myosin heavy chain IIb mediates herpes simplex virus 1 entry. J Virol. 2014; 89(3):1879-88. PMC: 4300746. DOI: 10.1128/JVI.03079-14. View

17.

Zhang Q, Yoo D . PRRS virus receptors and their role for pathogenesis. Vet Microbiol. 2015; 177(3-4):229-41. DOI: 10.1016/j.vetmic.2015.04.002. View

18.

Das P, Saha S, Chandra S, Das A, Dey S, Das M . Phosphorylation of Nonmuscle myosin II-A regulatory light chain resists Sendai virus fusion with host cells. Sci Rep. 2015; 5:10395. PMC: 4438666. DOI: 10.1038/srep10395. View

19.

Xiong D, Du Y, Wang H, Zhao B, Zhang H, Li Y . Nonmuscle myosin heavy chain IIA mediates Epstein-Barr virus infection of nasopharyngeal epithelial cells. Proc Natl Acad Sci U S A. 2015; 112(35):11036-41. PMC: 4568263. DOI: 10.1073/pnas.1513359112. View

20.

Whitworth K, Rowland R, Ewen C, Trible B, Kerrigan M, Cino-Ozuna A . Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus. Nat Biotechnol. 2015; 34(1):20-2. DOI: 10.1038/nbt.3434. View