Construction of Supramolecular Nanotubes from Protein Crystals

Overview

Journal Chem Sci

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2019 Feb 19

PMID 30774900

Citations 8

Authors

Tien Khanh Nguyen

Hashiru Negishi

Satoshi Abe

Takafumi Ueno

Affiliations

Soon will be listed here.

Abstract

Investigations involving the design of protein assemblies for the development of biomaterials are receiving significant attention. In nature, proteins can be driven into assemblies frequently by various non-covalent interactions. Assembly of proteins into supramolecules can be conducted under limited conditions in solution. These factors force the assembly process into an equilibrium state with low stability. Here, we report a new method for preparing assemblies using protein crystals as non-equilibrium molecular scaffolds. Protein crystals provide an ideal environment with a highly ordered packing of subunits in which the supramolecular assembled structures are formed in the crystalline matrix. Based on this feature, we demonstrate the self-assembly of supramolecular nanotubes constructed from protein crystals triggered by co-oxidation with cross-linkers. The assembly of tubes is driven by the formation of disulfide bonds to retain the intermolecular interactions within each assembly in the crystalline matrix after dissolution of the crystals.

Citing Articles

Coupling of Spectrin Repeat Modules for the Assembly of Nanorods and Presentation of Protein Domains.

Mezgec K, Snoj J, Ulcakar L, Ljubetic A, Znidaric M, Skarabot M ACS Nano. 2024; 18(42):28748-28763.

PMID: 39392430 PMC: 11503911. DOI: 10.1021/acsnano.4c07701.

A disulphide bond-mediated hetero-dimer of a hemoprotein and a fluorescent protein exhibiting efficient energy transfer.

Soon J, Oohora K, Hayashi T RSC Adv. 2022; 12(44):28519-28524.

PMID: 36320522 PMC: 9535469. DOI: 10.1039/d2ra05249k.

Creation of Cross-Linked Crystals With Intermolecular Disulfide Bonds Connecting Symmetry-Related Molecules Allows Retention of Tertiary Structure in Different Solvent Conditions.

Hiromoto T, Ikura T, Honjo E, Blaber M, Kuroki R, Tamada T Front Mol Biosci. 2022; 9:908394.

PMID: 35755825 PMC: 9213883. DOI: 10.3389/fmolb.2022.908394.

Protein Assembly by Design.

Zhu J, Avakyan N, Kakkis A, Hoffnagle A, Han K, Li Y Chem Rev. 2021; 121(22):13701-13796.

PMID: 34405992 PMC: 9148388. DOI: 10.1021/acs.chemrev.1c00308.

A Supramolecular Assembly of Hemoproteins Formed in a Star-Shaped Structure via Heme-Heme Pocket Interactions.

Soon J, Oohora K, Hirayama S, Hayashi T Int J Mol Sci. 2021; 22(3).

PMID: 33498330 PMC: 7864044. DOI: 10.3390/ijms22031012.

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