Mitotic Chromosome Alignment Ensures Mitotic Fidelity by Promoting Interchromosomal Compaction During Anaphase

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2019 Feb 9

PMID 30733233

Citations 44

Authors

Cindy L Fonseca

Heidi L H Malaby

Leslie A Sepaniac

Whitney Martin

Candice Byers

Anne Czechanski

Dana Messinger

Mary Tang

Ryoma Ohi

Laura G Reinholdt

Jason Stumpff

Affiliations

Soon will be listed here.

Abstract

Chromosome alignment at the equator of the mitotic spindle is a highly conserved step during cell division; however, its importance to genomic stability and cellular fitness is not understood. Normal mammalian somatic cells lacking KIF18A function complete cell division without aligning chromosomes. These alignment-deficient cells display normal chromosome copy numbers in vitro and in vivo, suggesting that chromosome alignment is largely dispensable for maintenance of euploidy. However, we find that loss of chromosome alignment leads to interchromosomal compaction defects during anaphase, abnormal organization of chromosomes into a single nucleus at mitotic exit, and the formation of micronuclei in vitro and in vivo. These defects slow cell proliferation and are associated with impaired postnatal growth and survival in mice. Our studies support a model in which the alignment of mitotic chromosomes promotes proper organization of chromosomes into a single nucleus and continued proliferation by ensuring that chromosomes segregate as a compact mass during anaphase.

Citing Articles

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