Secondary-Ion Mass Spectrometry Images Cardiolipins and Phosphatidylethanolamines at the Subcellular Level

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2019 Jan 26

PMID 30680861

Citations 32

Authors

Hua Tian

Louis J Sparvero

Paul Blenkinsopp

Andrew A Amoscato

Simon C Watkins

Hulya Bayir

Valerian E Kagan

Nicholas Winograd

Affiliations

Soon will be listed here.

Abstract

Millions of diverse molecules constituting the lipidome act as important signals within cells. Of these, cardiolipin (CL) and phosphatidylethanolamine (PE) participate in apoptosis and ferroptosis, respectively. Their subcellular distribution is largely unknown. Imaging mass spectrometry is capable of deciphering the spatial distribution of multiple lipids at subcellular levels. Here we report the development of a unique 70 keV gas-cluster ion beam that consists of (CO ) (n>10 000) projectiles. Coupled with direct current beam buncher-time-of-flight secondary-ion mass spectrometry, it is optimized for sensitivity towards high-mass species (up to m/z 3000) at high spatial resolution (1 μm). In combination with immunohistochemistry, phospholipids, including PE and CL, have been assessed in subcellular compartments of mouse hippocampal neuronal cells and rat brain tissue.

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