Using Mass Spectrometry Imaging to Visualize Age-related Subcellular Disruption

Overview

Journal Front Mol Biosci

Specialty Biology

Date 2023 Mar 27

PMID 36968274

Authors

Kelly A Hogan

Julianna D Zeidler

Heather K Beasley

Abrar I Alsaadi

Abdulkareem A Alshaheeb

Yi-Chin Chang

Hua Tian

Antentor O Hinton Jr

Melanie R McReynolds

Affiliations

Soon will be listed here.

Abstract

Metabolic homeostasis balances the production and consumption of energetic molecules to maintain active, healthy cells. Cellular stress, which disrupts metabolism and leads to the loss of cellular homeostasis, is important in age-related diseases. We focus here on the role of organelle dysfunction in age-related diseases, including the roles of energy deficiencies, mitochondrial dysfunction, endoplasmic reticulum (ER) stress, changes in metabolic flux in aging (e.g., Ca and nicotinamide adenine dinucleotide), and alterations in the endoplasmic reticulum-mitochondria contact sites that regulate the trafficking of metabolites. Tools for single-cell resolution of metabolite pools and metabolic flux in animal models of aging and age-related diseases are urgently needed. High-resolution mass spectrometry imaging (MSI) provides a revolutionary approach for capturing the metabolic states of individual cells and cellular interactions without the dissociation of tissues. mass spectrometry imaging can be a powerful tool to elucidate the role of stress-induced cellular dysfunction in aging.

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