14-3-3ζ Promotes Gliomas Cells Invasion by Regulating Snail Through the PI3K/AKT Signaling

Overview

Journal Cancer Med

Specialty Oncology

Date 2019 Jan 19

PMID 30656845

Citations 21

Authors

Junjun Li

Hao Xu

Qiangping Wang

Sihua Wang

Nanxiang Xiong

Affiliations

Soon will be listed here.

Abstract

14-3-3ζ has been reported to function as critical regulators of diverse cellular responses. However, the role of 14-3-3ζ in gliomas progression remains largely unknown. The expression level of 14-3-3ζ and Snail was detected by Western blot analysis and quantitative polymerase chain reaction in different grades of human gliomas. The effect of 14-3-3ζ on gliomas progression was measured using cell migration and invasion assay, the colony formation experiment, and CCK-8 assay. The effect of 14-3-3ζ on PI3K/AKT/Snail signaling protein expression levels was tested by Western blotting. Firstly, 14-3-3ζ was often up-regulated in high-grade gliomas relative to low-grade gliomas, and this overexpression was significantly related to tumor size, Karnofsky Performance Scale score and weaker disease-free survival. Secondly, the overexpression of 14-3-3ζ promoted gliomas cells proliferation, migration, and invasion. Conversely, the knockdown of 14-3-3ζ suppressed gliomas cells proliferation, migration, and invasion. Furthermore, subsequent mechanistic studies showed that 14-3-3ζ could activate PI3K/AKT/Snail signaling pathway to facilitate gliomas cells proliferation, migration, and invasion. This study shows that the overexpression of 14-3-3ζ can promote remarkably gliomas cells proliferation, migration, and invasion by regulating the Snail protein expression through activating PI3K/AKT signaling, and it may serve as a potential prognostic marker and therapeutic target for gliomas.

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References

Li J, Xu H, Wang Q, Wang S, Xiong N . 14-3-3ζ promotes gliomas cells invasion by regulating Snail through the PI3K/AKT signaling. Cancer Med. 2019; 8(2):783-794. PMC: 6382716. DOI: 10.1002/cam4.1950. View

Engelman J . Targeting PI3K signalling in cancer: opportunities, challenges and limitations. Nat Rev Cancer. 2009; 9(8):550-62. DOI: 10.1038/nrc2664. View

Tzivion G, Gupta V, Kaplun L, Balan V . 14-3-3 proteins as potential oncogenes. Semin Cancer Biol. 2006; 16(3):203-13. DOI: 10.1016/j.semcancer.2006.03.004. View

Louis D, Ohgaki H, Wiestler O, Cavenee W, Burger P, Jouvet A . The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol. 2007; 114(2):97-109. PMC: 1929165. DOI: 10.1007/s00401-007-0243-4. View

Aitken A . 14-3-3 proteins: a historic overview. Semin Cancer Biol. 2006; 16(3):162-72. DOI: 10.1016/j.semcancer.2006.03.005. View

Benzinger A, Muster N, Koch H, Yates 3rd J, Hermeking H . Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer. Mol Cell Proteomics. 2005; 4(6):785-95. DOI: 10.1074/mcp.M500021-MCP200. View

Yu J, Li J, Chen Y, Cao W, Lu Y, Yang J . Snail Enhances Glycolysis in the Epithelial-Mesenchymal Transition Process by Targeting FBP1 in Gastric Cancer. Cell Physiol Biochem. 2017; 43(1):31-38. DOI: 10.1159/000480314. View

Peinado H, Olmeda D, Cano A . Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype?. Nat Rev Cancer. 2007; 7(6):415-28. DOI: 10.1038/nrc2131. View

Myung J, Cho B, Kim Y, Park S . Snail and Cox-2 expressions are associated with WHO tumor grade and survival rate of patients with gliomas. Neuropathology. 2009; 30(3):224-31. DOI: 10.1111/j.1440-1789.2009.01072.x. View

10.

Liu M, Cai S, Espejo A, Bedford M, Walker C . 14-3-3 interacts with the tumor suppressor tuberin at Akt phosphorylation site(s). Cancer Res. 2002; 62(22):6475-80. View

11.

Aitken A . 14-3-3 proteins on the MAP. Trends Biochem Sci. 1995; 20(3):95-7. DOI: 10.1016/s0968-0004(00)88971-9. View

12.

Caja L, Tzavlaki K, S Dadras M, Tan E, Hatem G, Maturi N . Snail regulates BMP and TGFβ pathways to control the differentiation status of glioma-initiating cells. Oncogene. 2018; 37(19):2515-2531. PMC: 5945579. DOI: 10.1038/s41388-018-0136-0. View

13.

Jin J, Smith F, Stark C, Wells C, Fawcett J, Kulkarni S . Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization. Curr Biol. 2004; 14(16):1436-50. DOI: 10.1016/j.cub.2004.07.051. View

14.

Lacroix M, Abi-Said D, Fourney D, Gokaslan Z, Shi W, Demonte F . A multivariate analysis of 416 patients with glioblastoma multiforme: prognosis, extent of resection, and survival. J Neurosurg. 2002; 95(2):190-8. DOI: 10.3171/jns.2001.95.2.0190. View

15.

Tzivion G, Avruch J . 14-3-3 proteins: active cofactors in cellular regulation by serine/threonine phosphorylation. J Biol Chem. 2001; 277(5):3061-4. DOI: 10.1074/jbc.R100059200. View

16.

Mohammadi A, Hawasli A, Rodriguez A, Schroeder J, Laxton A, Elson P . The role of laser interstitial thermal therapy in enhancing progression-free survival of difficult-to-access high-grade gliomas: a multicenter study. Cancer Med. 2014; 3(4):971-9. PMC: 4303165. DOI: 10.1002/cam4.266. View

17.

Pozuelo Rubio M, Geraghty K, Wong B, Wood N, Campbell D, Morrice N . 14-3-3-affinity purification of over 200 human phosphoproteins reveals new links to regulation of cellular metabolism, proliferation and trafficking. Biochem J. 2004; 379(Pt 2):395-408. PMC: 1224091. DOI: 10.1042/BJ20031797. View

18.

Guancial E, Werner L, Bellmunt J, Bamias A, Choueiri T, Ross R . FGFR3 expression in primary and metastatic urothelial carcinoma of the bladder. Cancer Med. 2014; 3(4):835-44. PMC: 4303151. DOI: 10.1002/cam4.262. View

19.

Porter G, Khuri F, Fu H . Dynamic 14-3-3/client protein interactions integrate survival and apoptotic pathways. Semin Cancer Biol. 2006; 16(3):193-202. DOI: 10.1016/j.semcancer.2006.03.003. View

20.

Cao W, Kawai N, Miyake K, Zhang X, Fei Z, Tamiya T . Relationship of 14-3-3zeta (ζ), HIF-1α, and VEGF expression in human brain gliomas. Brain Tumor Pathol. 2013; 31(1):1-10. DOI: 10.1007/s10014-013-0135-3. View