Anti-melanogenesis and Anti-tyrosinase Properties of Subsp. Extracts on B16F10 Murine Melanoma Cells

Overview

Journal Res Pharm Sci

Specialty Chemistry

Date 2019 Jan 5

PMID 30607151

Citations 17

Authors

Samira Eghbali-Feriz

Akram Taleghani

Hadi Al-Najjar

Seyed Ahmad Emami

Homa Rahimi

Javad Asili

Samira Hasanzadeh

Zahra Tayarani-Najaran

Affiliations

Soon will be listed here.

Abstract

() subsp. has been used in traditional medicine and is famous for its medicinal properties. The aim of this study was to evaluate the effect of methanol (MeOH), n-hexane, dichloromethane (CHCl), n-butanol (BuOH), ethyl acetate (EtOAc), water extracts and essential oil of subsp. on melanin synthesis and oxidative stress in B16F10 melanoma cell line. The B16F10 cells viability after treatment with increasing concentrations of different extracts of the plant (0.2-200 μg/mL) was measured using resazurin. Essential oil composition was identified by gas-chromatography-mass spectrometry (GC-MS) analysis and inhibitory effect on synthesis of melanin, mushroom tyrosinase activity, cellular tyrosinase, and oxidative stress were evaluated by the colorimetric and fluorometric methods. The data showed extracts at concentrations 0.2-200 μg/mL, did not show significant toxicity on melanoma cells but concentrations of 200 μg/mL of essential oil had cytotoxic effect. subsp. could inhibit the mushroom tyrosinase activity. Also the amount of melanin in B16F10 cells declined. In addition, the ability of subsp. extracts in decreasing the amount of reactive oxygen species in melanoma cells revealed remarkable antioxidant activity. In addition, all concentrations of essential oil had no significant effect in this study. The melanogenesis inhibitory and antioxidant effects of subsp. on B16F10 cells may suggest the potential whitening activity of the plant for using in dermatological skin care products and for prevention of skin aging in cosmetic industry.

Citing Articles

Investigating the effect of cGRP78 vaccine against different cancer cells and its role in reducing melanoma metastasis.

Zare H, Bakherad H, Esfahani A, Aghamollaei H, Mousavi Gargari S, Aliomrani M Res Pharm Sci. 2024; 19(1):73-82.

PMID: 39006979 PMC: 11244710. DOI: 10.4103/1735-5362.394822.

Preparation and characterization of novel nanostructured lipid carriers (NLC) and solid lipid nanoparticles (SLN) containing coenzyme Q10 as potent antioxidants and antityrosinase agents.

Atapour-Mashhad H, Tayarani-Najaran Z, Golmohammadzadeh S Heliyon. 2024; 10(11):e31429.

PMID: 38882272 PMC: 11180323. DOI: 10.1016/j.heliyon.2024.e31429.

Evaluation of the cytotoxic activities of the essential oil from Pistacia atlantica Desf. oleoresin on human gastric cancer cell lines.

Saeedi F, Salehi M, Kamali M, Aghajani Mir M, Kazemi S, Shirafkan F Med Oncol. 2024; 41(6):148.

PMID: 38733486 DOI: 10.1007/s12032-024-02339-z.

Span 60/Cholesterol Niosomal Formulation as a Suitable Vehicle for Gallic Acid Delivery with Potent In Vitro Antibacterial, Antimelanoma, and Anti-Tyrosinase Activity.

Zolghadri S, Asad A, Farzi F, Ghajarzadeh F, Habibi Z, Rahban M Pharmaceuticals (Basel). 2023; 16(12).

PMID: 38139807 PMC: 10748266. DOI: 10.3390/ph16121680.

Searching for Natural Plants with Antimelanogenesis and Antityrosinase Properties for Cosmeceutical or Nutricosmetics Applications: A Systematic Review.

Tung X, Yip J, Gew L ACS Omega. 2023; 8(37):33115-33201.

PMID: 37744793 PMC: 10515176. DOI: 10.1021/acsomega.3c02994.

References

OBrien J, Wilson I, Orton T, Pognan F . Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem. 2000; 267(17):5421-6. DOI: 10.1046/j.1432-1327.2000.01606.x. View

Kim Y, Yun J, Lee C, Lee H, Min K, Kim Y . Oxyresveratrol and hydroxystilbene compounds. Inhibitory effect on tyrosinase and mechanism of action. J Biol Chem. 2002; 277(18):16340-4. DOI: 10.1074/jbc.M200678200. View

Chen Q, Kubo I . Kinetics of mushroom tyrosinase inhibition by quercetin. J Agric Food Chem. 2002; 50(14):4108-12. DOI: 10.1021/jf011378z. View

Riley P . Melanogenesis and melanoma. Pigment Cell Res. 2003; 16(5):548-52. DOI: 10.1034/j.1600-0749.2003.00069.x. View

Malkia A, Murtomaki L, Urtti A, Kontturi K . Drug permeation in biomembranes: in vitro and in silico prediction and influence of physicochemical properties. Eur J Pharm Sci. 2004; 23(1):13-47. DOI: 10.1016/j.ejps.2004.05.009. View

Agar N, Young A . Melanogenesis: a photoprotective response to DNA damage?. Mutat Res. 2005; 571(1-2):121-32. DOI: 10.1016/j.mrfmmm.2004.11.016. View

Ortonne J, Bissett D . Latest insights into skin hyperpigmentation. J Investig Dermatol Symp Proc. 2008; 13(1):10-4. DOI: 10.1038/jidsymp.2008.7. View

Choi M, Song H, Hur H, Sim S . Whitening activity of luteolin related to the inhibition of cAMP pathway in alpha-MSH-stimulated B16 melanoma cells. Arch Pharm Res. 2008; 31(9):1166-71. DOI: 10.1007/s12272-001-1284-4. View

Shan J, Fu J, Zhao Z, Kong X, Huang H, Luo L . Chlorogenic acid inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in RAW264.7 cells through suppressing NF-kappaB and JNK/AP-1 activation. Int Immunopharmacol. 2009; 9(9):1042-8. DOI: 10.1016/j.intimp.2009.04.011. View

10.

Abdel-Daim M, Funasaka Y, Komoto M, Nakagawa Y, Yanagita E, Nishigori C . Pharmacogenomics of metabotropic glutamate receptor subtype 1 and in vivo malignant melanoma formation. J Dermatol. 2010; 37(7):635-46. DOI: 10.1111/j.1346-8138.2010.00833.x. View

11.

Ye Y, Chou G, Wang H, Chu J, Yu Z . Flavonoids, apigenin and icariin exert potent melanogenic activities in murine B16 melanoma cells. Phytomedicine. 2010; 18(1):32-5. DOI: 10.1016/j.phymed.2010.06.004. View

12.

Si Y, Yin S, Oh S, Wang Z, Ye S, Yan L . An integrated study of tyrosinase inhibition by rutin: progress using a computational simulation. J Biomol Struct Dyn. 2012; 29(5):999-1012. DOI: 10.1080/073911012010525028. View

13.

Chung K, Park Y, Choi Y, Park M, Ha Y, Uehara Y . Evaluation of in vitro and in vivo anti-melanogenic activity of a newly synthesized strong tyrosinase inhibitor (E)-3-(2,4 dihydroxybenzylidene)pyrrolidine-2,5-dione (3-DBP). Biochim Biophys Acta. 2012; 1820(7):962-9. DOI: 10.1016/j.bbagen.2012.03.018. View

14.

Huang H, Hsieh W, Niu Y, Chang T . Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract. BMC Complement Altern Med. 2012; 12:72. PMC: 3404006. DOI: 10.1186/1472-6882-12-72. View

15.

Qiao Z, Koizumi Y, Zhang M, Natsui M, Flores M, Gao L . Anti-melanogenesis effect of Glechoma hederacea L. extract on B16 murine melanoma cells. Biosci Biotechnol Biochem. 2012; 76(10):1877-83. DOI: 10.1271/bbb.120341. View

16.

Rezaei P, Fouladdel S, Ghaffari S, Amin G, Azizi E . Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells. Daru. 2013; 20(1):101. PMC: 3556048. DOI: 10.1186/2008-2231-20-101. View

17.

Tayarani-Najaran Z, Mousavi S, Tajfard F, Asili J, Soltani S, Hatamipour M . Cytotoxic and apoptogenic properties of three isolated diterpenoids from Salvia chorassanica through bioassay-guided fractionation. Food Chem Toxicol. 2013; 57:346-51. DOI: 10.1016/j.fct.2013.03.037. View

18.

Saghaie L, Pourfarzam M, Fassihi A, Sartippour B . Synthesis and tyrosinase inhibitory properties of some novel derivatives of kojic acid. Res Pharm Sci. 2013; 8(4):233-42. PMC: 3757588. View

19.

Bozorgi M, Memariani Z, Mobli M, Salehi Surmaghi M, Shams-Ardekani M, Rahimi R . Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): a review of their traditional uses, phytochemistry, and pharmacology. ScientificWorldJournal. 2014; 2013:219815. PMC: 3876903. DOI: 10.1155/2013/219815. View

20.

Boozari M, Mohammadi A, Asili J, Emami S, Tayarani-Najaran Z . Growth inhibition and apoptosis induction by Scutellaria pinnatifida A. Ham. on HL-60 and K562 leukemic cell lines. Environ Toxicol Pharmacol. 2014; 39(1):307-12. DOI: 10.1016/j.etap.2014.12.002. View