Cytotoxicity Assay of D-limonene Niosomes: an Efficient Nano-carrier for Enhancing Solubility of Plant-extracted Agents

Overview

Journal Res Pharm Sci

Specialty Chemistry

Date 2019 Dec 5

PMID 31798662

Citations 40

Authors

Mohammad Reza Hajizadeh

Haniyeh Maleki

Mahmood Barani

Mohammad Ali Fahmidehkar

Mehdi Mahmoodi

Masoud Torkzadeh-Mahani

Affiliations

Soon will be listed here.

Abstract

The low solubility of the plant-extracted agent like D-limonene in cancer therapy is a critical problem. In this study, we prepared D-limonene-loaded niosomes (D-limonene/Nio) for cancer therapy through cytotoxicity assay of HepG2, MCF-7, and A549 cell lines. The niosomal formulation was prepared by film hydration technique with Span 40: Tween 40: cholesterol (35:35:30 molar ratio) and characterized for vesicle distribution size, morphology, entrapment efficiency (EE%), and release behaviour. The obtained niosomes showed a nanometric size and spherical morphology with EE% about 87 ± 1.8%. Remarkably prolonged release of D-limonene from niosomes compared to free D-limonene observed. The loaded formulation showed significantly enhanced cytotoxic activity with all three cancer cell lines (HepG2, Macf-7 and A549) at the concentration of 20 μM. These results indicated that niosome loaded with phytochemicals can be a promising nano-carrier for cancer therapy applications.

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References

Sun J . D-Limonene: safety and clinical applications. Altern Med Rev. 2007; 12(3):259-64. View

Jing L, Zhang Y, Fan S, Gu M, Guan Y, Lu X . Preventive and ameliorating effects of citrus D-limonene on dyslipidemia and hyperglycemia in mice with high-fat diet-induced obesity. Eur J Pharmacol. 2013; 715(1-3):46-55. DOI: 10.1016/j.ejphar.2013.06.022. View

Cui F, Shi K, Zhang L, Tao A, Kawashima Y . Biodegradable nanoparticles loaded with insulin-phospholipid complex for oral delivery: preparation, in vitro characterization and in vivo evaluation. J Control Release. 2006; 114(2):242-50. DOI: 10.1016/j.jconrel.2006.05.013. View

Abbasi S, Kajimoto K, Harashima H . Critical parameters dictating efficiency of membrane-mediated drug transfer using nanoparticles. Int J Pharm. 2018; 553(1-2):398-407. DOI: 10.1016/j.ijpharm.2018.10.042. View

Arnold M, Soerjomataram I, Ferlay J, Forman D . Global incidence of oesophageal cancer by histological subtype in 2012. Gut. 2014; 64(3):381-7. DOI: 10.1136/gutjnl-2014-308124. View

Hariharan S, Bhardwaj V, Bala I, Sitterberg J, Bakowsky U, Kumar M . Design of estradiol loaded PLGA nanoparticulate formulations: a potential oral delivery system for hormone therapy. Pharm Res. 2005; 23(1):184-95. DOI: 10.1007/s11095-005-8418-y. View

Murali R, Karthikeyan A, Saravanan R . Protective effects of D-limonene on lipid peroxidation and antioxidant enzymes in streptozotocin-induced diabetic rats. Basic Clin Pharmacol Toxicol. 2012; 112(3):175-81. DOI: 10.1111/bcpt.12010. View

Liu R . Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr. 2003; 78(3 Suppl):517S-520S. DOI: 10.1093/ajcn/78.3.517S. View

Yu X, Lin H, Wang Y, Lv W, Zhang S, Qian Y . d-limonene exhibits antitumor activity by inducing autophagy and apoptosis in lung cancer. Onco Targets Ther. 2018; 11:1833-1847. PMC: 5894671. DOI: 10.2147/OTT.S155716. View

10.

Attia I, El-Gizawy S, Fouda M, Donia A . Influence of a niosomal formulation on the oral bioavailability of acyclovir in rabbits. AAPS PharmSciTech. 2008; 8(4):E106. PMC: 2750692. DOI: 10.1208/pt0804106. View

11.

Davarpanah F, Yazdi A, Barani M, Mirzaei M, Torkzadeh-Mahani M . Magnetic delivery of antitumor carboplatin by using PEGylated-Niosomes. Daru. 2018; . PMC: 6154485. DOI: 10.1007/s40199-018-0215-3. View

12.

Guyton K, Kensler T . Prevention of liver cancer. Curr Oncol Rep. 2002; 4(6):464-70. DOI: 10.1007/s11912-002-0057-4. View

13.

Thakkar M, Brijesh S . Physicochemical investigation and in vivo activity of anti-malarial drugs co-loaded in Tween 80 niosomes. J Liposome Res. 2017; 28(4):315-321. DOI: 10.1080/08982104.2017.1376684. View

14.

Behroozeh A, Mazloumi Tabrizi M, Kazemi S, Choupani E, Kabiri N, Ilbeigi D . Evaluation the Anti-Cancer Effect of PEGylated Nano-Niosomal Gingerol, on Breast Cancer Cell lines (T47D), In-Vitro. Asian Pac J Cancer Prev. 2018; 19(3):645-648. PMC: 5980835. DOI: 10.22034/APJCP.2018.19.3.645. View

15.

Lazutka J, Mierauskiene J, Slapsyte G, Dedonyte V . Genotoxicity of dill (Anethum graveolens L.), peppermint (Menthaxpiperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. Food Chem Toxicol. 2001; 39(5):485-92. DOI: 10.1016/s0278-6915(00)00157-5. View

16.

Alexis F, Rhee J, Richie J, Radovic-Moreno A, Langer R, Farokhzad O . New frontiers in nanotechnology for cancer treatment. Urol Oncol. 2008; 26(1):74-85. DOI: 10.1016/j.urolonc.2007.03.017. View

17.

Barani M, Mirzaei M, Torkzadeh-Mahani M, Nematollahi M . Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer. Daru. 2018; 26(1):11-17. PMC: 6154483. DOI: 10.1007/s40199-018-0207-3. View

18.

Rajera R, Nagpal K, Singh S, Mishra D . Niosomes: a controlled and novel drug delivery system. Biol Pharm Bull. 2011; 34(7):945-53. DOI: 10.1248/bpb.34.945. View

19.

Hemati M, Haghiralsadat F, Yazdian F, Jafari F, Moradi A, Malekpour-Dehkordi Z . Development and characterization of a novel cationic PEGylated niosome-encapsulated forms of doxorubicin, quercetin and siRNA for the treatment of cancer by using combination therapy. Artif Cells Nanomed Biotechnol. 2018; 47(1):1295-1311. DOI: 10.1080/21691401.2018.1489271. View

20.

Li W, Lian Y, Guan Q, Li N, Liang W, Liu W . Liver-targeted delivery of liposome-encapsulated curcumol using galactosylated-stearate. Exp Ther Med. 2018; 16(2):925-930. PMC: 6090458. DOI: 10.3892/etm.2018.6210. View