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Mitochondrial DNA Variations in Tongue Squamous Cell Carcinoma

Overview
Journal Biomed Rep
Specialty Biochemistry
Date 2018 Dec 28
PMID 30588299
Citations 5
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Abstract

Tongue squamous cell carcinoma (TSCC) is the most common type of oral carcinoma. Mitochondrial DNA (mtDNA) is a circular DNA molecule of 16,569 bp, which functionally encompasses a regulatory non-coding region (D-loop) and 37 encoding genes that correspond to 13 subunits of respiratory chain complexes (I, III, IV and V), 22 transfer RNAs and 2 ribosomal (r)RNAs. Recently, mtDNA has been implicated as a mutation hotspot in various tumors. However, to our knowledge mtDNA alteration in TSCC has not been investigated to date. In the present study, the mitochondrial genomes of tongue carcinoma, adjacent non-cancerous tissue and peripheral blood samples from 8 patients with TSCC were sequenced and aligned with the revised Cambridge Reference Sequence. Overall, only one synonymous mutation, which mapped to the NADH:ubiquinone oxidoreductase core subunit 5 gene, was observed in the tongue carcinoma sample from a single patient. A further 21 polymorphisms were identified, including six in the non-coding region (D-loop), five in Complex I, three in Complex III, two in Complex IV, two in Complex V and three in rRNA. In addition, mitochondrial microsatellite instability (mtMSI) was detected in 2/8 tongue carcinoma samples, and localized in the D310 region. These variations, particularly the polymorphisms and mtMSI, imply that the mitochondrial genome may be a hotspot of genome alteration in tongue cancer. Further investigation is expected to reveal the role of mtDNA alteration in TSCC development, as well as its clinical implications.

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References
1.
CSORDAS G, Hajnoczky G . Sorting of calcium signals at the junctions of endoplasmic reticulum and mitochondria. Cell Calcium. 2001; 29(4):249-62. DOI: 10.1054/ceca.2000.0191. View

2.
Penta J, Johnson F, Wachsman J, Copeland W . Mitochondrial DNA in human malignancy. Mutat Res. 2001; 488(2):119-33. DOI: 10.1016/s1383-5742(01)00053-9. View

3.
Djouadi F, Bonnefont J, Munnich A, Bastin J . Characterization of fatty acid oxidation in human muscle mitochondria and myoblasts. Mol Genet Metab. 2003; 78(2):112-8. DOI: 10.1016/s1096-7192(03)00017-9. View

4.
Matsuyama W, Nakagawa M, Wakimoto J, Hirotsu Y, Kawabata M, Osame M . Mitochondrial DNA mutation correlates with stage progression and prognosis in non-small cell lung cancer. Hum Mutat. 2003; 21(4):441-3. DOI: 10.1002/humu.10196. View

5.
Pedersen P . Tumor mitochondria and the bioenergetics of cancer cells. Prog Exp Tumor Res. 1978; 22:190-274. DOI: 10.1159/000401202. View