EnvZ, a Transmembrane Environmental Sensor of Escherichia Coli K-12, is Phosphorylated in Vitro

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 Dec 1

PMID 3056929

Citations 68

Authors

M M Igo

T J Silhavy

Affiliations

Soon will be listed here.

Abstract

By fusing the transcriptional and translational start signals of lacZ to envZ, we have obtained high-level synthesis of a truncated EnvZ protein (EnvZ115) in which the first 38 amino acids of EnvZ are replaced with the first 8 amino acids of LacZ. Using this construct, we have partially purified the EnvZ115 protein and demonstrated that this protein can be phosphorylated in vitro. We suggest that phosphorylation may be an important feature of EnvZ function.

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