Crosstalk Between Bacterial Chemotaxis Signal Transduction Proteins and Regulators of Transcription of the Ntr Regulon: Evidence That Nitrogen Assimilation and Chemotaxis Are Controlled by a Common Phosphotransfer Mechanism

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Aug 1

PMID 3041412

Citations 74

Authors

A J Ninfa

E G Ninfa

A N Lupas

A Stock

B MAGASANIK

J Stock

Affiliations

Soon will be listed here.

Abstract

We demonstrate by using purified bacterial components that the protein kinases that regulate chemotaxis and transcription of nitrogen-regulated genes, CheA and NRII, respectively, have cross-specificities: CheA can phosphorylate the Ntr transcription factor NRI and thereby activate transcription from the nitrogen-regulated glnA promoter, and NRII can phosphorylate CheY. In addition, we find that a high intracellular concentration of a highly active mutant form of NRII can suppress the smooth-swimming phenotype of a cheA mutant. These results argue strongly that sensory transduction in the Ntr and Che systems involves a common protein phosphotransfer mechanism.

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