Dietary Ingestion of Calories and Micronutrients Modulates the DNA Methylation Profile of Leukocytes from Older Individuals

Overview

Journal J Nutr Health Aging

Specialties Geriatrics
Nutritional Sciences

Date 2018 Dec 1

PMID 30498838

Citations 3

Authors

J Passador

L V Toffoli

K B Fernandes

R D Neves-Souza

G G Pelosi

M V Gomes

Affiliations

Soon will be listed here.

Abstract

Objective: Several lines of evidence from the last decade support the connection between nutrition and epigenetic mechanisms. In the present study we evaluated the impact of the daily dietary intake of calories and the micronutrients vitamin A, D, B1, B2, B5, C, E, copper, calcium, phosphorus, iron, iodine, selenium, manganese, potassium and sodium on the global DNA methylation profile of blood cells from older individuals.

Research Methods And Procedures: The study enrolled 126 physically independent elderly of both sexes (60 men and 66 women). For the molecular analysis, DNA samples were extracted from leukocytes and global DNA methylation was evaluated using a high throughput Elisa-based method. Correlations between global DNA methylation and the daily intake of calorie or micronutrients were evaluated using Prism5 GraphPad Software.

Results: A statistically significant correlation was observed between global DNA methylation and the daily caloric value (p=0.019, r=-0.21), and the intake of vitamin A (p=0.03, r=-0.18), Vitamin E (p=0.027, r=-0.20) and copper (p=0.04, r=-0.18). No correlation was observed between global DNA methylation and the daily intake of vitamin D, B1, B2, B5, C, calcium, phosphorus, iron, iodine, selenium, manganese and potassium (p>0.05).

Conclusion: Our data demonstrate that the daily intake of calories or the micronutrients vitamin A, vitamin E and copper can potentially modulate the global DNA methylation profile of leukocytes in older adults and corroborate the notion of nutritional influences on epigenetic mechanisms.

Citing Articles

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References

Kandi V, Vadakedath S . Effect of DNA Methylation in Various Diseases and the Probable Protective Role of Nutrition: A Mini-Review. Cureus. 2015; 7(8):e309. PMC: 4582005. DOI: 10.7759/cureus.309. View

Slimani N, Deharveng G, Charrondiere R, van Kappel A, Ocke M, Welch A . Structure of the standardized computerized 24-h diet recall interview used as reference method in the 22 centers participating in the EPIC project. European Prospective Investigation into Cancer and Nutrition. Comput Methods Programs Biomed. 1999; 58(3):251-66. DOI: 10.1016/s0169-2607(98)00088-1. View

Munteanu A, Zingg J, Azzi A . Anti-atherosclerotic effects of vitamin E--myth or reality?. J Cell Mol Med. 2004; 8(1):59-76. PMC: 6740318. DOI: 10.1111/j.1582-4934.2004.tb00260.x. View

Pufulete M, Al-Ghnaniem R, Leather A, Appleby P, Gout S, Terry C . Folate status, genomic DNA hypomethylation, and risk of colorectal adenoma and cancer: a case control study. Gastroenterology. 2003; 124(5):1240-8. DOI: 10.1016/s0016-5085(03)00279-8. View

Davis S, Davis B, Richens J, Vere K, Petrov P, Winlove C . α-Tocopherols modify the membrane dipole potential leading to modulation of ligand binding by P-glycoprotein. J Lipid Res. 2015; 56(8):1543-50. PMC: 4513995. DOI: 10.1194/jlr.M059519. View

Palmieri C, Rudraraju B, Monteverde M, Lattanzio L, Gojis O, Brizio R . Methylation of the calcium channel regulatory subunit α2δ-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas. Br J Cancer. 2012; 107(2):375-81. PMC: 3394973. DOI: 10.1038/bjc.2012.231. View

Pallavi R, Giorgio M, Pelicci P . Insights into the beneficial effect of caloric/ dietary restriction for a healthy and prolonged life. Front Physiol. 2012; 3:318. PMC: 3429088. DOI: 10.3389/fphys.2012.00318. View

Gomes M, Toffoli L, Arruda D, Soldera L, Pelosi G, Neves-Souza R . Age-related changes in the global DNA methylation profile of leukocytes are linked to nutrition but are not associated with the MTHFR C677T genotype or to functional capacities. PLoS One. 2013; 7(12):e52570. PMC: 3527598. DOI: 10.1371/journal.pone.0052570. View

Fuke C, Shimabukuro M, Petronis A, Sugimoto J, Oda T, Miura K . Age related changes in 5-methylcytosine content in human peripheral leukocytes and placentas: an HPLC-based study. Ann Hum Genet. 2004; 68(Pt 3):196-204. DOI: 10.1046/j.1529-8817.2004.00081.x. View

10.

Hou L, Wang H, Sartori S, Gawron A, Lissowska J, Bollati V . Blood leukocyte DNA hypomethylation and gastric cancer risk in a high-risk Polish population. Int J Cancer. 2010; 127(8):1866-74. PMC: 3009461. DOI: 10.1002/ijc.25190. View

11.

Penarrubia L, Romero P, Carrio-Segui A, Andres-Borderia A, Moreno J, Sanz A . Temporal aspects of copper homeostasis and its crosstalk with hormones. Front Plant Sci. 2015; 6:255. PMC: 4400860. DOI: 10.3389/fpls.2015.00255. View

12.

Brigelius-Flohe R, Kelly F, Salonen J, Neuzil J, Zingg J, Azzi A . The European perspective on vitamin E: current knowledge and future research. Am J Clin Nutr. 2002; 76(4):703-16. DOI: 10.1093/ajcn/76.4.703. View

13.

de Jong N, Mulder I, de Graaf C, van Staveren W . Impaired sensory functioning in elders: the relation with its potential determinants and nutritional intake. J Gerontol A Biol Sci Med Sci. 1999; 54(8):B324-31. DOI: 10.1093/gerona/54.8.b324. View

14.

Joseph J, Cole G, Head E, Ingram D . Nutrition, brain aging, and neurodegeneration. J Neurosci. 2009; 29(41):12795-801. PMC: 6665319. DOI: 10.1523/JNEUROSCI.3520-09.2009. View

15.

Sadeghi N, Oveisi M, Jannat B, Hajimahmoodi M, Behzad M, Behfar A . The relationship between bone health and plasma zinc, copper lead and cadmium concentration in osteoporotic women. J Environ Health Sci Eng. 2014; 12(1):125. PMC: 4251679. DOI: 10.1186/s40201-014-0125-3. View

16.

Esteller M . Epigenetics in cancer. N Engl J Med. 2008; 358(11):1148-59. DOI: 10.1056/NEJMra072067. View

17.

Portela A, Esteller M . Epigenetic modifications and human disease. Nat Biotechnol. 2010; 28(10):1057-68. DOI: 10.1038/nbt.1685. View

18.

Olerup O, ZETTERQUIST H . HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens. 1992; 39(5):225-35. DOI: 10.1111/j.1399-0039.1992.tb01940.x. View

19.

Hsiung D, Marsit C, Houseman E, Eddy K, Furniss C, McClean M . Global DNA methylation level in whole blood as a biomarker in head and neck squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev. 2007; 16(1):108-14. DOI: 10.1158/1055-9965.EPI-06-0636. View

20.

Lim U, Flood A, Choi S, Albanes D, Cross A, Schatzkin A . Genomic methylation of leukocyte DNA in relation to colorectal adenoma among asymptomatic women. Gastroenterology. 2008; 134(1):47-55. PMC: 2211566. DOI: 10.1053/j.gastro.2007.10.013. View