Mouse Models of Accelerated Cellular Senescence

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2018 Nov 27

PMID 30474850

Citations 27

Authors

Matthew J Yousefzadeh

Kendra I Melos

Luise Angelini

Christin E Burd

Paul D Robbins

Laura J Niedernhofer

Affiliations

Soon will be listed here.

Abstract

Senescent cells accumulate in multiple tissues as virtually all vertebrate organisms age. Senescence is a highly conserved response to many forms of cellular stress intended to block the propagation of damaged cells. Senescent cells have been demonstrated to play a causal role in aging via their senescence-associated secretory phenotype and by impeding tissue regeneration. Depletion of senescent cells either through genetic or pharmacologic methods has been demonstrated to extend murine lifespan and delay the onset of age-related diseases. Measuring the burden and location of senescent cells in vivo remains challenging, as there is no marker unique to senescent cells. Here, we describe multiple methods to detect the presence and extent of cellular senescence in preclinical models, with a special emphasis on murine models of accelerated aging that exhibit a more rapid onset of cellular senescence.

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