Cancellation of Bessel Beam Side Lobes for High-contrast Light Sheet Microscopy

Overview

Journal Sci Rep

Specialty Science

Date 2018 Nov 23

PMID 30464219

Citations 9

Authors

Giuseppe Di Domenico

Giancarlo Ruocco

Cristina Colosi

Eugenio DelRe

Giuseppe Antonacci

Affiliations

Soon will be listed here.

Abstract

An ideal illumination for light sheet fluorescence microscopy entails both a localized and a propagation invariant optical field. Bessel beams and Airy beams satisfy these conditions, but their non-diffracting feature comes at the cost of the presence of high-energy side lobes that notably degrade the imaging contrast and induce photobleaching. Here, we demonstrate the use of a light droplet illumination whose side lobes are suppressed by interfering Bessel beams of specific k-vectors. Our droplet illumination readily achieves more than 50% extinction of the light distributed across the Bessel side lobes, providing a more efficient energy localization without loss in transverse resolution. In a standard light sheet fluorescence microscope, we demonstrate a two-fold contrast enhancement imaging micron-scale fluorescent beads. Results pave the way to new opportunities for rapid and deep in vivo observations of large-scale biological systems.

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References

Keller P, Schmidt A, Santella A, Khairy K, Bao Z, Wittbrodt J . Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy. Nat Methods. 2010; 7(8):637-42. PMC: 4418465. DOI: 10.1038/nmeth.1476. View

Dodt H, Leischner U, Schierloh A, Jahrling N, Mauch C, Deininger K . Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain. Nat Methods. 2007; 4(4):331-6. DOI: 10.1038/nmeth1036. View

Andilla J, Jorand R, Olarte O, Dufour A, Cazales M, Montagner Y . Imaging tissue-mimic with light sheet microscopy: A comparative guideline. Sci Rep. 2017; 7:44939. PMC: 5381005. DOI: 10.1038/srep44939. View

He F, Yu J, Tan Y, Chu W, Zhou C, Cheng Y . Tailoring femtosecond 1.5-μm Bessel beams for manufacturing high-aspect-ratio through-silicon vias. Sci Rep. 2017; 7:40785. PMC: 5241677. DOI: 10.1038/srep40785. View

Chen B, Legant W, Wang K, Shao L, Milkie D, Davidson M . Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution. Science. 2014; 346(6208):1257998. PMC: 4336192. DOI: 10.1126/science.1257998. View

Gao L . Extend the field of view of selective plan illumination microscopy by tiling the excitation light sheet. Opt Express. 2015; 23(5):6102-11. DOI: 10.1364/OE.23.006102. View

Keller P, Schmidt A, Wittbrodt J, Stelzer E . Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy. Science. 2008; 322(5904):1065-9. DOI: 10.1126/science.1162493. View

Asuncion A, Guerrero R . Generating superimposed Bessel beams with a volume holographic axicon. Appl Opt. 2017; 56(14):4206-4212. DOI: 10.1364/AO.56.004206. View

Fahrbach F, Gurchenkov V, Alessandri K, Nassoy P, Rohrbach A . Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation. Opt Express. 2013; 21(11):13824-39. DOI: 10.1364/OE.21.013824. View

10.

Gao L, Shao L, Higgins C, Poulton J, Peifer M, Davidson M . Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens. Cell. 2012; 151(6):1370-85. PMC: 3615549. DOI: 10.1016/j.cell.2012.10.008. View

11.

Ruffner D, Grier D . Optical conveyors: a class of active tractor beams. Phys Rev Lett. 2012; 109(16):163903. DOI: 10.1103/PhysRevLett.109.163903. View

12.

Conchello J, Lichtman J . Optical sectioning microscopy. Nat Methods. 2005; 2(12):920-31. DOI: 10.1038/nmeth815. View

13.

Welf E, Driscoll M, Dean K, Schafer C, Chu J, Davidson M . Quantitative Multiscale Cell Imaging in Controlled 3D Microenvironments. Dev Cell. 2016; 36(4):462-75. PMC: 4784259. DOI: 10.1016/j.devcel.2016.01.022. View

14.

Buytaert J, Dirckx J . Design and quantitative resolution measurements of an optical virtual sectioning three-dimensional imaging technique for biomedical specimens, featuring two-micrometer slicing resolution. J Biomed Opt. 2007; 12(1):014039. DOI: 10.1117/1.2671712. View

15.

Breuninger T, Greger K, Stelzer E . Lateral modulation boosts image quality in single plane illumination fluorescence microscopy. Opt Lett. 2007; 32(13):1938-40. DOI: 10.1364/ol.32.001938. View

16.

Di Domenico G, Parravicini J, Antonacci G, Silvestri S, Agranat A, DelRe E . Miniaturized photogenerated electro-optic axicon lens Gaussian-to-Bessel beam conversion. Appl Opt. 2017; 56(10):2908-2911. DOI: 10.1364/AO.56.002908. View

17.

Olarte O, Licea-Rodriguez J, Palero J, Gualda E, Artigas D, Mayer J . Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles. Biomed Opt Express. 2012; 3(7):1492-505. PMC: 3395476. DOI: 10.1364/BOE.3.001492. View

18.

Ahrens M, Orger M, Robson D, Li J, Keller P . Whole-brain functional imaging at cellular resolution using light-sheet microscopy. Nat Methods. 2013; 10(5):413-20. DOI: 10.1038/nmeth.2434. View

19.

Zhang P, Phipps M, Goodwin P, Werner J . Confocal line scanning of a Bessel beam for fast 3D imaging. Opt Lett. 2014; 39(12):3682-5. DOI: 10.1364/OL.39.003682. View

20.

Golub I, Chebbi B, Golub J . Toward the optical "magic carpet": reducing the divergence of a light sheet below the diffraction limit. Opt Lett. 2015; 40(21):5121-4. DOI: 10.1364/OL.40.005121. View