Imaging Tissue-mimic with Light Sheet Microscopy: A Comparative Guideline

Overview

Journal Sci Rep

Specialty Science

Date 2017 Mar 22

PMID 28322312

Citations 15

Authors

Jordi Andilla

Raphael Jorand

Omar E Olarte

Alexandre C Dufour

Martine Cazales

Yoann L E Montagner

Romain Ceolato

Nicolas Riviere

Jean-Christophe Olivo-Marin

Pablo Loza-Alvarez

Corinne Lorenzo

Affiliations

Soon will be listed here.

Abstract

Tissue mimics (TMs) on the scale of several hundred microns provide a beneficial cell culture configuration for in vitro engineered tissue and are currently under the spotlight in tissue engineering and regenerative medicine. Due to the cell density and size, TMs are fairly inaccessible to optical observation and imaging within these samples remains challenging. Light Sheet Fluorescence Microscopy (LSFM)- an emerging and attractive technique for 3D optical sectioning of large samples- appears to be a particularly well-suited approach to deal with them. In this work, we compared the effectiveness of different light sheet illumination modalities reported in the literature to improve resolution and/or light exposure for complex 3D samples. In order to provide an acute and fair comparative assessment, we also developed a systematic, computerized benchmarking method. The outcomes of our experiment provide meaningful information for valid comparisons and arises the main differences between the modalities when imaging different types of TMs.

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