High-throughput Volumetric Mapping of Synaptic Transmission

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2024 Jun 19

PMID 38898094

Authors

Wei Chen

Xinxin Ge

Qinrong Zhang

Ryan G Natan

Jiang Lan Fan

Massimo Scanziani

Na Ji

Affiliations

Soon will be listed here.

Abstract

Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. Applying our method to imaging glutamate release, we demonstrated high-throughput mapping of excitatory inputs at >1,000 synapses per volume and >500 dendritic spines per neuron in vivo and unveiled previously unseen features of functional synaptic organization in the mouse primary visual cortex.

Citing Articles

In Vivo Neurodynamics Mapping via High-Speed Two-Photon Fluorescence Lifetime Volumetric Projection Microscopy.

Li Y, Xu X, Zhang C, Sun X, Zhou S, Li X Adv Sci (Weinh). 2024; 12(7):e2410605.

PMID: 39716869 PMC: 11831470. DOI: 10.1002/advs.202410605.

Multiphoton fluorescence microscopy for in vivo imaging.

Xu C, Nedergaard M, Fowell D, Friedl P, Ji N Cell. 2024; 187(17):4458-4487.

PMID: 39178829 PMC: 11373887. DOI: 10.1016/j.cell.2024.07.036.

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