Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2018 Nov 8

PMID 30400456

Citations 14

Authors

Ismail Hussain Kamal Basha

Eric Tatt Wei Ho

Caffiyar Mohamed Yousuff

Nor Hisham Bin Hamid

Affiliations

Soon will be listed here.

Abstract

Highly sensitive and specific pathogen diagnosis is essential for correct and timely treatment of infectious diseases, especially virulent strains, in people. Point-of-care pathogen diagnosis can be a tremendous help in managing disease outbreaks as well as in routine healthcare settings. Infectious pathogens can be identified with high specificity using molecular methods. A plethora of microfluidic innovations in recent years have now made it increasingly feasible to develop portable, robust, accurate, and sensitive genomic diagnostic devices for deployment at the point of care. However, improving processing time, multiplexed detection, sensitivity and limit of detection, specificity, and ease of deployment in resource-limited settings are ongoing challenges. This review outlines recent techniques in microfluidic genomic diagnosis and devices with a focus on integrating them into a lab on a chip that will lead towards the development of multiplexed point-of-care devices of high sensitivity and specificity.

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References

Henihan G, Schulze H, Corrigan D, Giraud G, Terry J, Hardie A . Label- and amplification-free electrochemical detection of bacterial ribosomal RNA. Biosens Bioelectron. 2016; 81:487-494. DOI: 10.1016/j.bios.2016.03.037. View

Compton J . Nucleic acid sequence-based amplification. Nature. 1991; 350(6313):91-2. DOI: 10.1038/350091a0. View

Liu W, Zhang M, Liu X, Sharma A, Ding X . A Point-of-Need infrared mediated PCR platform with compatible lateral flow strip for HPV detection. Biosens Bioelectron. 2017; 96:213-219. DOI: 10.1016/j.bios.2017.04.047. View

Kim T, Park J, Kim C, Cho Y . Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens. Anal Chem. 2014; 86(8):3841-8. DOI: 10.1021/ac403971h. View

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N . Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28(12):E63. PMC: 102748. DOI: 10.1093/nar/28.12.e63. View

Comina G, Suska A, Filippini D . Towards autonomous lab-on-a-chip devices for cell phone biosensing. Biosens Bioelectron. 2015; 77:1153-67. DOI: 10.1016/j.bios.2015.10.092. View

Sun Y, Quyen T, Hung T, Chin W, Wolff A, Bang D . A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples. Lab Chip. 2015; 15(8):1898-904. DOI: 10.1039/c4lc01459f. View

Collins M, Irvine B, Tyner D, Fine E, Zayati C, Chang C . A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml. Nucleic Acids Res. 1997; 25(15):2979-84. PMC: 146852. DOI: 10.1093/nar/25.15.2979. View

Cheng Y, Wang Y, Wang Z, Huang L, Bi M, Xu W . A mechanical cell disruption microfluidic platform based on an on-chip micropump. Biomicrofluidics. 2017; 11(2):024112. PMC: 5533499. DOI: 10.1063/1.4979100. View

10.

Shen F, Davydova E, Du W, Kreutz J, Piepenburg O, Ismagilov R . Digital isothermal quantification of nucleic acids via simultaneous chemical initiation of recombinase polymerase amplification reactions on SlipChip. Anal Chem. 2011; 83(9):3533-40. PMC: 3101872. DOI: 10.1021/ac200247e. View

11.

Rohrman B, Richards-Kortum R . A paper and plastic device for performing recombinase polymerase amplification of HIV DNA. Lab Chip. 2012; 12(17):3082-8. PMC: 3569001. DOI: 10.1039/c2lc40423k. View

12.

Wimbles R, Melling L, Shaw K . Combining Electro-Osmotic Flow and FTA Paper for DNA Analysis on Microfluidic Devices. Micromachines (Basel). 2018; 7(7). PMC: 6190317. DOI: 10.3390/mi7070119. View

13.

Borysiak M, Kimura K, Posner J . NAIL: Nucleic Acid detection using Isotachophoresis and Loop-mediated isothermal amplification. Lab Chip. 2015; 15(7):1697-707. DOI: 10.1039/c4lc01479k. View

14.

Hsieh K, Patterson A, Ferguson B, Plaxco K, Soh H . Rapid, sensitive, and quantitative detection of pathogenic DNA at the point of care through microfluidic electrochemical quantitative loop-mediated isothermal amplification. Angew Chem Int Ed Engl. 2012; 51(20):4896-900. PMC: 3509743. DOI: 10.1002/anie.201109115. View

15.

Houston K, Tucker M, Chowdhury J, Shirley N, Little A . The Plant Cell Wall: A Complex and Dynamic Structure As Revealed by the Responses of Genes under Stress Conditions. Front Plant Sci. 2016; 7:984. PMC: 4978735. DOI: 10.3389/fpls.2016.00984. View

16.

Escobedo C, Burgel S, Kemmerling S, Sauter N, Braun T, Hierlemann A . On-chip lysis of mammalian cells through a handheld corona device. Lab Chip. 2015; 15(14):2990-7. DOI: 10.1039/c5lc00552c. View

17.

Mullis K, Faloona F, Scharf S, Saiki R, Horn G, Erlich H . Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol. 1986; 51 Pt 1:263-73. DOI: 10.1101/sqb.1986.051.01.032. View

18.

Vincent M, Xu Y, Kong H . Helicase-dependent isothermal DNA amplification. EMBO Rep. 2004; 5(8):795-800. PMC: 1249482. DOI: 10.1038/sj.embor.7400200. View

19.

Dobnik D, Stebih D, Blejec A, Morisset D, Zel J . Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection. Sci Rep. 2016; 6:35451. PMC: 5064307. DOI: 10.1038/srep35451. View

20.

Bissonnette L, Bergeron M . Infectious Disease Management through Point-of-Care Personalized Medicine Molecular Diagnostic Technologies. J Pers Med. 2015; 2(2):50-70. PMC: 4251365. DOI: 10.3390/jpm2020050. View