Effect of Microfluidic Processing on the Viability of Boar and Bull Spermatozoa

Overview

Journal Biomicrofluidics

Publisher American Institute of Physics

Specialty Biomedical Engineering

Date 2020 Aug 11

PMID 32774586

Citations 1

Authors

Tanja Hamacher

Johanna T W Berendsen

Stella A Kruit

Marleen L W J Broekhuijse

Loes I Segerink

Affiliations

Soon will be listed here.

Abstract

The use of microfluidics in artificial reproductive technologies for manipulation or assessment of spermatozoa is unique in the sense that it is not always an end point measurement and the sample may be used afterward. During microfluidic processing, spermatozoa are exposed to shear stress, which may harm viability and functioning of spermatozoa. The shear stresses during general microfluidic processing steps were calculated and compared to estimated shear stresses during ejaculation. The viability of boar and bull spermatozoa after microfluidic processing was studied and compared to the typical handling method (centrifugation) and to a control (the sample in a tube at the same temperature). The boar spermatozoa showed a small but significant decrease in viability of 6% after microfluidic handling. Bull spermatozoa proved to be less susceptible to shear stress and were not significantly affected by microfluidic processing. These data indicate that the impact of microfluidic processing on the viability of boar and bull spermatozoa is less than the literature values reported for flow cytometry and comparable to the impact of centrifugation.

Citing Articles

An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section II.

Orsolini M, Meyers S, Dini P Animals (Basel). 2021; 11(11).

PMID: 34828049 PMC: 8614388. DOI: 10.3390/ani11113319.

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