Extraction of Astaxanthin and Lutein from Microalga in the Red Phase Using CO₂ Supercritical Fluid Extraction Technology with Ethanol As Co-Solvent

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2018 Nov 8

PMID 30400304

Citations 31

Authors

Antonio Molino

Sanjeet Mehariya

Angela Iovine

Vincenzo Larocca

Giuseppe Di Sanzo

Maria Martino

Patrizia Casella

Simeone Chianese

Dino Musmarra

Affiliations

Soon will be listed here.

Abstract

Astaxanthin and lutein, antioxidants used in nutraceutics and cosmetics, can be extracted from several microalgal species. In this work, investigations on astaxanthin and lutein extraction from () in the red phase were carried out by means of the supercritical fluid extraction (SFE) technique, in which CO₂ supercritical fluid was used as the extracting solvent with ethanol as the co-solvent. The experimental activity was performed using a bench-scale reactor in semi-batch configuration with varying extraction times (20, 40, 60, and 80 min), temperatures (50, 65, and 80 °C) and pressures (100, 400, and 550 bar). Moreover, the performance of CO₂ SFE with ethanol was compared to that without ethanol. The results show that the highest astaxanthin and lutein recoveries were found at 65 °C and 550 bar, with ~18.5 mg/g dry weight (~92%) astaxanthin and ~7.15 mg/g dry weight (~93%) lutein. The highest astaxanthin purity and the highest lutein purity were found at 80 °C and 400 bar, and at 65 °C and 550 bar, respectively.

Citing Articles

Intensification of the SFE Using Ethanol as a Cosolvent and Integration of the SFE Process with sc-CO Followed by PLE Using Pressurized Ethanol of Black Soldier Fly ( L.) Larvae Meal-Extract Yields and Characterization.

Cruz V, Ferreira N, Le Roux E, Destandau E, de Oliveira A Foods. 2024; 13(11).

PMID: 38890848 PMC: 11171942. DOI: 10.3390/foods13111620.

Techno-economic analysis of a new downstream process for the production of astaxanthin from the microalgae Haematococcus pluvialis.

Bauer A, Minceva M Bioresour Bioprocess. 2024; 8(1):111.

PMID: 38650201 PMC: 10992946. DOI: 10.1186/s40643-021-00463-6.

Sc-CO extraction of fish and fish by-products in the production of fish oil and enzyme.

Jamalluddin N, Ismail N, Mutalib S, Sikin A Bioresour Bioprocess. 2024; 9(1):21.

PMID: 38647764 PMC: 10992331. DOI: 10.1186/s40643-022-00509-3.

A New Method for Selective Extraction of Torularhodin from Red Yeast Using CO-SFE Technique.

Ambrico A, Larocca V, Trupo M, Martino M, Magarelli R, Spagnoletta A Appl Biochem Biotechnol. 2024; 196(9):6473-6491.

PMID: 38386146 PMC: 11604813. DOI: 10.1007/s12010-024-04884-9.

Extraction and Purification of Highly Active Astaxanthin from Fermentation Broth.

Seeger J, Wendisch V, Henke N Mar Drugs. 2023; 21(10).

PMID: 37888465 PMC: 10608131. DOI: 10.3390/md21100530.

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