Longitudinal Personal DNA Methylome Dynamics in a Human with a Chronic Condition

Overview

Journal Nat Med

Specialties General Medicine
Molecular Biology

Date 2018 Nov 7

PMID 30397358

Citations 32

Authors

Rui Chen

Lin Xia

Kailing Tu

Meixue Duan

Kimberly Kukurba

Jennifer Li-Pook-Than

Dan Xie

Michael Snyder

Affiliations

Soon will be listed here.

Abstract

Epigenomics regulates gene expression and is as important as genomics in precision personal health, as it is heavily influenced by environment and lifestyle. We profiled whole-genome DNA methylation and the corresponding transcriptome of peripheral blood mononuclear cells collected from a human volunteer over a period of 36 months, generating 28 methylome and 57 transcriptome datasets. We found that DNA methylomic changes are associated with infrequent glucose level alteration, whereas the transcriptome underwent dynamic changes during events such as viral infections. Most DNA meta-methylome changes occurred 80-90 days before clinically detectable glucose elevation. Analysis of the deep personal methylome dataset revealed an unprecedented number of allelic differentially methylated regions that remain stable longitudinally and are preferentially associated with allele-specific gene regulation. Our results revealed that changes in different types of 'omics' data associate with different physiological aspects of this individual: DNA methylation with chronic conditions and transcriptome with acute events.

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